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| Research on total polyphenol content of different ethanol fractions from Xanthoceras sorbifolia husks and the antioxidant activity |
| LI Guoqian1，2 QIN Tian3 HUANG Rong1，2 TANG Xiang1，2 YANG Chunyan1，2 ZHOU Chunyang1，2 |
1.School of Pharmacy, North Sichuan Medical College, Sichuan Province, Nanchong 637000, China;
2.Institute of Materia Medica, North Sichuan Medical College, Sichuan Province, Nanchong 637000, China;
3.Department of Preventive Medicine, North Sichuan Medical College, Sichuan Province, Nanchong 637000, China |
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Abstract Objective To establish the determination method of total polyphenol content (TPC) of the ethanol extract from X. sorbifolia husks, and evaluate the TPC and antioxidant activity in vitro of different ethanol fractions from X. sorbifolia husks. Methods The TPC of X. sorbifolia husks was analyzed by spectrophotometry. The antioxidant activity of different ethanol fractions from X. sorbifolia husks was evaluated by ABTS and DPPH methods. Results The TPC of X. sorbifolia husks was 10.22 mg/g. After purified by D101 macroporous resin, the polyphenols from X. sorbifolia husks were mainly concentrated in the 30% ethanol fraction, the TPC of which was 4.92 mg/g. This faction exhibited strong antioxidant activity, the IC50 values against ABTS and DPPH free radicals of which were (4.83±0.33), (9.33±0.23) μg/mL respectively. While the IC50 values of the positive control rutin against ABTS and DPPH free radicals were (12.87±0.21), (10.29±0.17) μg/mL respectively. Conclusion The 30% ethanol fraction purified by D101 macroporous resin has the highest TPC and the strongest antioxidant activity, which is worthy of further development and utilization.
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