高危型HPV E6/E7 mRNA联合液基细胞学检查提高宫颈病变检出率的研究

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Abstract Objective To study the application value of the high-risk human papillomavirus (HPV) E6/E7 mRNA combined with liquid based cytology in the improvement of detection rate of cervical lesion. Methods From January 2016 to December 2017, 249 patients with cervical lesions in Yan′an People′s Hospital were selected as the subjects. There were 170 cases of cervical inflammation, 32 cases of cervical intraepithelial neoplasia Ⅰ (CIN Ⅰ), 28 cases of CIN Ⅱ, 14 cases of CIN Ⅲ, 5 cases of cervical cancer. The detection of high-risk HPV E6/E7 mRNA, liquid based cytology and histopathological biopsy were carried among all patients. The detection rate, sensitivity, and specificity of different examination methods were compared with the results of pathological biopsy. Results The detection rate of high risk type HPV E6/E7 mRNA examination in the diagnosis of cervical inflammation, CIN was 83.53%, 71.62%, which was significantly higher than liquid based cytology of 68.24%, 56.76%, and was significantly lower than the joint detection of 94.71% and 90.54% respectively, the differences between groups were statistically significant (P < 0.05). The detection rate of high-risk type HPV E6/E7 mRNA examination in the diagnosis of CIN Ⅰwas 68.75%, which was significantly higher than the liquid based cytology of 43.75%, the joint detection was significantly lower than the 90.63%, the differences between groups were statistically significant (P < 0.05). The sensitivity of the high-risk HPV E6/E7 mRNA examination and the diagnosis of precancerous lesions was 76.60% and 68.09% respectively, which was significantly lower than 91.49% of the joint detection. The specificity of high-risk HPV E6/E7 mRNA in diagnosing precancerous lesions was 81.19%, which was significantly higher than the liquid based cytology of 64.36%, was significantly lower than the joint detection of 94.06%, the differences between groups were statistically significant (P < 0.05). Conclusion The high risk HPV E6/E7 mRNA combined with cytological examination can significantly improve the detection rate of cervical lesions, which has higher clinical diagnostic value, and worth popularizing.

Key words： Cervical lesions Human papillomavirus of high-risk type Cytological examination of liquid base Cervical intraepithelial neoplasia

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	MA Na ZHANG Yan▲ QIAO Na

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MA Na ZHANG Yan▲ QIAO Na. Study on high-risk HPV E6 / E7 mRNA combined with liquid - based cytology to improve the detection rate of cervical lesions[J]. 中国医药导报, 2018, 15(32): 80-83.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I32/80

[1] Perri F，Longo F，Della Vittoria Scarpati G，et al. Metastatic HPV-related oropharyngeal carcinoma cured with chemoradiotherapy：importance of pretherapy biomolecular assessment [J]. Clin Case Rep，2017，6（1）：56-62.
[2] 冯树芳，段兆珺，潘正娟，等.HR-HPV、TCT检查联合阴道镜定位活检在宫颈癌前病变及宫颈癌筛查中的价值[J].医学信息，2016，29（8）：124-125.
[3] 于淼淼，杨露.高危型人乳头状瘤病毒DNA检测与液基薄层细胞学检查在宫颈癌癌前病变中的诊断价值比较[J].中国医学前沿杂志：电子版，2016，8（12）：75-78.
[4] 许惠惠，石卫武，徐玖飞，等.HPV基因分型在宫颈癌筛查风险分层管理中的价值[J].中华实验和临床病毒学杂志，2016，23（1）：14-18.
[5] 杨娟，关婷，蔡虹，等.高危型人乳头瘤病毒检测作为宫颈癌初筛手段的应用研究[J].中国计划生育和妇产科，2016，32（2）：32-35.
[6] 姚有娣，程易凡，王丹凤，等.宫颈液基薄层细胞学检测与人乳头瘤病毒检测对宫颈癌诊断的效用评价[J].中国性科学，2016，25（2）：44-47.
[7] 高华玲，尹德娥，杜杰，等.TCT和HR-HPV-DNA检测在宫颈癌和癌前病变筛查中的临床价值[J].标记免疫分析与临床，2017，24（10）：1115-1118.
[8] 董宇凤，高丽.HPV感染与宫颈病变的相关性及常见危险因素[J].中国性科学，2016，25（5）：35-37.
[9] 王艳君，张静.高危型HPV联合TCT在宫颈癌筛查中的价值研究[J].中国性科学，2017，26（12）：24-27.
[10] Paz-Zulueta M，álvarez-Paredes L，Rodríguez Díaz JC，et al. Prevalence of high-risk HPV genotypes，categorised by their quadrivalent and nine-valent HPV vaccination coverage，and the genotype association with high-grade lesions [J]. BMC Cancer，2018，18（1）：112-113.
[11] 祁可娜，孙铮.高危型HPV联合液基薄层细胞学检查在宫颈癌早期诊断中的价值[J].中国妇幼健康研究，2017， 28（7）：887-889.
[12] 王海瑞，廖光东，江宇，等.p16/mcm2双染在宫颈上皮内瘤变的诊断价值及其与高危型HPV感染的关系[J].中华流行病学杂志，2017，38（9）：1241-1245.
[13] 廖光东，康乐妮，李静，等.p16/微小染色体维持蛋白2和p16/Ki-67双重染色对高危型人乳头瘤病毒呈阳性妇女的宫颈病变筛查[J].中华妇幼临床医学杂志：电子版，2017，13（4）：425-431.
[14] 师晓艳，雷侠.宫颈液基细胞学检查与高危型HPV检测用于宫颈癌前病变并发感染早期筛查的对比研究[J].中国性科学，2017，26（1）：33-35.
[15] 范玲玲，黄修菊，刘新莲，等.hTERC、C-MYC基因检测在新疆维吾尔族妇女宫颈病变筛查中的意义[J].临床检验杂志，2017，35（3）：196-198.
[16] 王晓光，辛志峰，李娜，等.人乳头瘤病毒检测与薄层液基细胞学技术在宫颈癌筛查中的应用[J].现代妇产科进展，2016，25（2）：140-142.
[17] 徐萍，方筠，应春妹，等.高危型HPV DNA检测与细胞学检查用于宫颈疾病诊疗筛查的结果分析[J].检验医学，2016，31（6）：516-520.
[18] 叶丽君，喻长法，蔡莎莎，等.高危型人乳头瘤病毒E6/E7 mRNA联合液基细胞学检查在宫颈病变诊断中的应用[J].中国慢性病预防与控制，2017，25（7）：499-501.
[19] 徐瑛，高志斌，黄丽丽，等.基于数学模型的高危HPV E6/E7 mRNA定量分析在宫颈病变筛查中的作用[J].中国医学物理学杂志，2017，34（1）：76-79.
[20] 王丹，李一春，刘霞，等.人乳头瘤病毒E6/E7 mRNA表达联合液基薄层细胞学检测对宫颈癌早期筛查和诊断的意义[J].中国妇幼保健，2017，32（24）：6147-6150.
[21] 王玉兰，薛剑侠，李晓旭，等.高危型人乳头瘤病毒E6/E7 mRNA检测在宫颈癌中的研究进展[J].山西医药杂志，2017，46（7）：778-780.
[22] Hwang SH，Shin HY，Lee DO. A prospective pilot evaluation of vaginal and urine self-sampling for the Roche cobas 4800 HPV test for cervical cancer screening [J]. Sci Rep，2018，8（1）：9015-9016.
[23] Long W，Yang Z，Li X，et al. HPV-16，HPV-58，and HPV-33 are the most carcinogenic HPV genotypes in Southwestern China and their viral loads are associated with severity of premalignant lesions in the cervix [J]. Virol J，2018，15（1）：94-95.