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| Research on the protective effect of ferulic acid on the apoptosis of A375 cells induced by hydrogen peroxide |
| LI Wei ZHENG Kun QU Yan CHEN Lijuan WANG Jiazhi |
| Jiamusi College of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Jiamusi 154007, China |
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Abstract Objective To observe the effect of ferulic acid on the apoptosis of melanocyte induced by oxidative stress. Methods According to random number table, A375 cells were divided into five groups: control group, model group, and high, middle, low concentrations of ferulic acid groups. Cells in the control group were cultured normally, while peroxide hydrogen was added into the cultured A375 cells of model group and high, middle, low concentrations of ferulic acid groups at the final concentration of 0.1 mmol/L, duration for 4 hours. The cells were stained with fluorescent dye DAPI. The apoptosis of each group was observed by fluorescence microscope. Caspase active kit was used to measure the activity of Caspase in each group. Results Compared with the control group, the activities of Caspase-3, 8 and 9 in the model group were significantly increased, with statistically significant differences (P ＜ 0.01). In the model group, dense and concentrated particles and the loose light colored crescent or annular fluorescence could be seen in the nucleus and cytoplasm, which implied that hydrogen peroxide can induce apoptosis of A375 cells. Compared with the model group, the expression of Caspase-3 and Caspase-8 were significantly reduced in the high does concentration of ferulic acid group, with statistically significant differences (P < 0.05 or P ＜ 0.01), but the effect on Caspase-9 was not obvious. The nucleus of cells in the high does concentration of ferulic acid group were even bright blue, without the phenomenon of dense condensation. There were chromatin agglutination and marginalization of most cell nucleus in the low and medium does concentrations of ferulic acid groups. Conclusion Ferulic acid can protect the apoptosis of melanocyte through lower the activity of Caspase induced by hydrogen peroxide.
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