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| Primary research on the protective effect of formononetin on local cerebral ischemia-reperfusion injury in rats |
| JIANG Cijing WU Qiuhui REN Qianyao WANG Yong CHEN Jian LU Huiling▲ |
| Staff Room of Pathophysiology, Guilin Medical University, Guangxi Zhuang Autonomous Region, Guilin 541004, China |
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Abstract Objective To investigate the protective effect of formononetin on local cerebral ischemia-reperfusion injury in rats. Methods According to the random number table method, 42 male SD rats were divided into sham group, ischemia-reperfusion group and formononetin group, with 14 rats in each group. The formononetin group was given formononetin solution 20 mg/kg by intraperitoneal injection, the sham group and ischemia-reperfusion group were injected with same dose of normal saline, for 7 days. Suture-occluded method was used to copy the model of rats with cerebral ischemia-reperfusion injury in middle cerebral artery, the ischemic time was 1.5 h. After reperfusion for 24 h, the neurologic impairment of all groups was detected; the dry/wet method was used to detect the degree of cerebral edema; 2,3,5-tripheyltetrazolium (TTC) staining was used to calculate the volume of cerebral infarction and detect the activities of plasma superoxide dismutase (SOD) and the levels of malondialdehyde (MDA). Results Compared with ischemia-reperfusion group, the score of neurologic impairment in formononetin group was decreased, the volume of cerebral infarction of the second brain slice and the degree of cerebral edema of the third brain slice were reduced, the activities of plasma SOD were increased, and the levels of MDA were declined, the differences were all statistically significant (P ＜ 0.05 or P ＜ 0.01). Conclusion Formononetin can improve the degree of neurologic impairment, reduce the volume of cerebral infarction, the mechanism may be related to the decrease of oxidative stress.
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