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Study on the technology of enzyme-assisted homogenate extraction method for the extraction of procyanidins from seedpod of lotus |
WANG Jianmei GAO Hong XU Qiaona ZHANG Yuzhen YU Le ZHANG Shuning CHEN Fei |
Medical College, Jinhua Polytechnic, Zhejiang Province, Jinhua 321000, China |
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Abstract Objective To research on extraction process of proanthocyanidins from seedpod of lotus with homogenate extraction method. Methods Taking the proanthocyanidins yield of seedpod of lotus as the evaluation index, single factor test was used to investigate the effect of volume fraction of solvent extraction, ethanol extraction time, extraction times and solid-liquid ratio on the extraction of procyanidins from seedpod of lotus. The orthogonal tests of four factors and three levels were carried out with ethanol volume fraction, material liquid ratio, extraction time and blank as the object of study. The optimum extraction process parameters were determined. Results The results showed that the optimum process conditions were as followed, the volume fraction of ethanol was 60%, the extraction time was 3 min, and the liquid material ratio was 40∶1. Conclusion With this condition, the proanthocyanidins yield of seedpod of lotus is 8.12%. Compared with the traditional extraction method, the flash method has the advantages of high efficiency and less time.
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