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| Experimental study of autologous platelet-rich plasma in combination with calcium phosphate cement in repairing vertebral bone defect |
| GUO Ying1,2 JIA Lianshun2 WU Weimin3 ZHANG Lijun4 XING Guo1 PENG Bo5 WU Bo4 |
1.Department of Spine Surgery, No.187 Central Hospital of PLA, Hainan Province, Haikou 571159, China;
2.the Third Department of Spinal Surgery, Changzheng Hospital Affiliated to the Second Military Medical University, Shanghai 200003, China;
3.Department of General Surgery, No.187 Central Hospital of PLA, Hainan Province, Haikou 571159, China;
4.Department of Anesthesiology, No.187 Central Hospital of PLA, Hanian Province, Haikou 571159, China;
5.Department of Traumatic Orthopedics, No.187 Central Hospital of PLA, Hainan Province, Haikou 571159, China |
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Abstract Objective To observe the effect of the mixture of autologous platelet-rich plasma (PRP) and calcium phosphate cement (CPC) in repairing vertebral bone defect. Methods 18 goats were included in this experiment. A 6 mm×10 mm cylindrical bone defect was prepared on the side of lumbar vertebral body(L1-L6) of each sheep. The experiment was divided into three groups according to different filling methods: the experimental group was filled by CPC in combination with PRP, the control group was filled with CPC, and the sham operation group was not filled. 6 goats were randomly executed after surgery for 1, 3, 6 months, then the bone healing conditons were observed by imaging examination. Results All animals successfully completed surgery and survived. The platelet count in PRP was about 5 times more than that in whole blood, and the contents of growth factors [platelet derived growth factor (PDGF), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF)] in PRP were significantly higher than those in the whole blood (P ＜ 0.05). In all time points after surgery, new bone formation and cement absorption could be observed in both experimental group and control group, but the experimental group was more obvious, while no significant bone repair sign could be seen in sham operation group. Conclusion PRP can release a large number of growth factors, and show great properties of osteoinductive and osteogenesis when combined with CPC. The composite is effective for the treatment of vertebral bone defect.
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[1] Schmitz JP，Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions [J]. Clin Orthop Relat Res，1986，205（205）：299-308.
[2] Mah J，Hung J，Wang J，et al. The efficacy of various alloplastic bone grafts on the healing of rat calvarial defects [J]. Eur J Orthod，2004，26（5）：475-482.
[3] Blom EJ，Klein-Nulend J，Wolke JG，et al. Transforming growth factor-beta1 incorporation in an alpha-tricalcium phosphate/dicalcium phosphate dihydrate/tetracalcium phosphate monoxide cement：release characteristics and physicochemical properties [J]. Biomaterials，2002，23（4）：1261-1268.
[4] Tschon M，Fini M，Giardino R，et al. Lights and shadows concerning platelet products for musculoskeletal regeneration [J]. Front Biosci（Elite Ed），2011，1（3）：96-107.
[5] Kasten P，Vogel J，Geiger F，et al. The effect of platelet-rich plasma on healing in critical-size long-bone defects [J]. Biomaterials，2008，29（29）：3983-3992.
[6] Marx RE. Platelet-rich plasma（PRP）：what is PRP and what is not PRP? [J]. Implant Dent，2001，10（4）：225-228.
[7] Graziani F，Ivanovski S，Cei S，et al. The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts [J]. Clin Oral Implants Res，2006，17（2）：212-219.
[8] Mazzocca AD，McCarthy MB，Chowaniec DM，et al. Platelet-rich plasma differs according to preparation method and human variability [J]. J Bone Joint Surg Am，2012，94（4）：308-316.
[9] Bi L，Cheng W，Fan H，et al. Reconstruction of goat tibial defects using an injectable tricalcium phosphate/chitosan in combination with autologous platelet-rich plasma [J]. Biomaterials，2010，31（12）：3201-3211.
[10] Zhu Y，Yuan M，Meng HY，et al. Basic science and clinical application of platelet-rich plasma for cartilage defects and osteoarthritis：a review [J]. Osteoarthritis Cartilage，2013，21（11）：1627-1637.
[11] Cavallo C，Roffi A，Grigolo B，et al. Platelet-Rich Plasma：The Choice of Activation Method Affects the Release of Bioactive Molecules [J]. Biomed Res Int，2016，2016（1）：6591717.
[12] Fufa D，Shealy B，Jacobson M，et al. Activation of platelet-rich plasma using soluble type Ⅰ collagen [J]. J Oral Maxillofac Surg，2008，66（4）：684-690.
[13] Chen JC，Ko CL，Shih CJ，et al. Calcium phosphate bone cement with 10 wt% platelet-rich plasma in vitro and in vivo [J]. J Dent，2012，40（2）：114-122. |
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