裸花紫珠提取物对银屑病作用及机制研究

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Abstract Objective To determine the anti-psoriasis effect of Callicarpa nudiflora extract. Methods Sixty KM mouses were randomly divided into 5 groups by random number table (n = 12), including model group, Callicarpa nudiflora low-dose group, Callicarpa nudiflora middle-dose group, Callicarpa nudiflora high-dose group and positive control group. They were given to normal saline, Callicarpa nudiflora extract (0.6 g/kg or 1.2 g/kg or 2.4 g/kg) and 1 mg/kg methotrexate by gastric perfusion for seven days respectively. Lateral line scales of granular layer were detected by HE method. The expression of Caspase3, Cleaved-Caspase3, Bax and BCL-2 mRNA and protein were detected by Real-time PCR and Western blot. Results Lateral line scales of granular layer of Callicarpa nudiflora middle-dose and high-dose groups was increased than those in the model group (P < 0.05). The expression of the pro-apoptotic proteins Caspase3, Cleaved-Caspase3, Bax mRNA and protein in callicarpa nudiflora middle-dose and high-dose groups were up-regulated than those in the model group (P < 0.05), the expression of the anti-apoptotic protein BCL-2 mRNA and protein was down-regulated (P < 0.05). Conclusion The Callicarpa nudiflora extract can exert anti-psoriatic effect by inducing apoptosis.

Key words： Callicarpa nudiflora Psoriasis Granular layer Apoptotic

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BA Li′na. Study of effect and potential mechanism of Callicarpa nudiflora extract against psoriasis[J]. 中国医药导报, 2018, 15(21): 14-18.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I21/14

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