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| Evaluation of the laboratory results of patients with syphilis |
| GUO Jie* JIAO Bingxin* LIU Ying QU Pei XU Xinmin LI Juan |
| Laboratory Department, Beijing Ditan Hospital, Capital Medical University, Beijing 100013, China |
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Abstract Objective To investigate the value of different combinations of serological tests in the diagnosis of syphilis. Methods The serum of 20 325 patients visited Beijing Ditan Hospital from September 2016 to August 2017 were collected. Three methods including toluene red unheated serum test (TRUST), treponema pallidum gelatin agglutination test (TPPA) and fluorescent treponema pallidum antibody absorption test (FTA) were used to test the syphilis antibodies in the serum sample. Results Among the 20 325 patients, 4480 were diagnosed with treponema pallidum (TP) infection and among these patients with TPPA-positive, 3342 cases were positive for TRUST, accounted for 74.6%; 1138 cases were negative for TRUST, accounted for 25.4%. Among 3342 cases of TPPA-positive and TP-positive patients, 979 cases were positive for FTA-ABS-IgM, accounted for 29.3%. Among 1138 cases of TPPA-positive and TRUST-negative patients, 12 cases were positive for FTA-ABS-IgM, accounted for 1.05%. The positive rate of IgM antibody in 979 patients with syphilis was logarithmically correlated with different dilutions of the TRUST (R2 = 0.9351). Conclusion For laboratories that carried out syphilis serological tests, TPPA and TRUST should be carried out simultaneously. FTA-ABS-IgM was an effective complement for two other methods with TRUST-negative and TRUST persistent positive after treatment.
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