结直肠癌miR-200a与miR-92a表达水平及其与临床病理特征的关系

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Abstract Objective To analyze the expression of miR-200a and miR-92a in colorectal cancer and the relation of clinicopathological features. Methods The 60 patients with colorectal cancers in Affiliated Tumor Hospital, Xinjiang Medical University for surgical treatment from February 2011 to August 2012 were collected. And the 60 colorectal cancer tissue specimens and paracancerous tissues were achieved in patients with colorectal cancer respectively. The positive expression rates of miR-200a in colorectal cancer tissues and paracancerous tissues were detected by in situ hybridization technique. The expression levels of miR-92a in colorectal cancer tissues and paracancerous tissues were detected by real-time PCR. The relativities of the expression of miR-92a and miR-200a and the clinicopathological features were analyzed. Follow-up 5 years, all patients with colorectal cancers were divided into survival group and death group according to the survival status of patients. The expression levels of miR-92a and miR-200a were compared between two groups. Results The positive rate of miR-200a and the expression level of miR-92a in colorectal cancer tissues were significantly higher than in paracancerous tissues (all P ＜ 0.05). The positive expression rates of miR-200a in the colorectal cancer patients of the middle and high degree of differentiation and TNM stage Ⅰ-Ⅱ were significantly lower than those of the low degree of differentiation and TNM stage Ⅲ-Ⅳ, which were 40.00%(18/45) vs. 73.33%(11/15), 58.06%(18/31) vs. 86.21%(25/29) (all P ＜ 0.05). The relative expressions of miR-92a in colorectal cancer patients of the TNM stage Ⅰ-Ⅱ and without lymph node metastasis were significantly lower than those of the TNM stage Ⅲ-Ⅳ and lymph node metastasis, the differences were statistically significant (all P ＜ 0.05). Follow-up 5 years, the survival group had 21 cases and death group had 39 cases. The positive rate of miR-200a and the relative expression of miR-92a in survival group were significantly lower than death group, the differences were statistically significant (all P ＜ 0.05). Conclusion The expression levels of miR-200a and miR-92a are highly in colorectal cancer, and are closely associated with the severity and prognosis.

Key words： Colorectal cancer miR-200a miR-92a Clinicopathological features Prognosis evaluation

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	YANG Xinhui ZHAO Weimin DAReBai·Redati ZENG Xiangyue

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YANG Xinhui ZHAO Weimin DAReBai·Redati ZENG Xiangyue. The analysis of the expression of miR-200a and miR-92a and the relation of clinicopathological features in colorectal cancer[J]. 中国医药导报, 2018, 15(13): 63-66,75.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I13/63

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