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| Effects of health care moxibustion at guānyuán on serum and intestinal oxidation and inflammatory reaction in aged rats |
| OUYANG Xiali1 DUAN Haoru1 JIN Qi2 ZHAO Baixiao3 HAN Li3 LUO Xue3 ZHAO Siyu3 ZHANG Gaoyu3 ZHANG Yangyini3 CHENG Yuanyuan3 |
1.School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China;
2.Department of Cataract Eye Disease, Eye Hospital, China Academy of Chinese Medical Sciences, Beijing 100029, China;
3.School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China |
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Abstract Objective To observe the effects of health care moxibustion at guānyuán on serum and intestinal oxidation and inflammatory reaction in aged rats, and explore the possible mechanism of anti-aging. Methods According to the method of random number table, 12 normal aging female Wistar rats were divided into old group (six rats) and moxibustion group (six rats), and six young female Wistar rats were divided into young group. The abdominal hairs of rats of three groups with the diameter of guānyuán about 1.0 cm were shaved off. Young group and old group: the rats were grabbed and put into a special fixator without other intervention, 20 min/d, for 40 days. Moxibustion group: the rats were grabbed and put into a special fixator, the abdomen was aligned with the opening at the bottom of the fixator, and the ignited moxa strips were placed under the opening, aiming at the guānyuán of the rats. During moxibustion, the distance between the moxa strips and the rats was adjusted to keep the temperature basically constant. Moxibustion intervention for 20 min/d, for 40 days. The levels of SOD, MDA, IL-6, TNF-α, IL-1β and IL-10 in peripheral serum and intestine were detected. The microcirculation blood perfusion of guānyuán was measured by laser Doppler flow meter. Results Compared with young group, the serum and intestinal SOD levels in old group were decreased (P < 0.05 or P < 0.01), and the serum and intestinal MDA levels in old group were increased (P < 0.05 or P < 0.01). Compared with old group, the intestinal SOD level in moxibustion group was significantly increased (P < 0.01), and the intestinal MDA level in moxibustion group was significantly decreased (P < 0.01). Compared with young group, the expression levels of IL-6, TNF-α and IL-10 in old group were increased (P < 0.05). Compared with old group, the expression levels of IL-6, TNF-α, IL-1β and IL-10 in moxibustion group were significantly decreased (P < 0.01). Compared with young group, the peak and amplitude of microcirculation of guānyuán in old group decreased, and the blood perfusion volume decreased significantly. Compared with old group, the peak and amplitude of microcirculation of guānyuán in moxibustion group increased, and the blood perfusion volume increased significantly. Conclusion Moxibustion at guānyuán can regulate the intestinal immune system and enhance the antioxidant capacity of naturally aging rats. The mechanism may be related to reducing the level of inflammatory cytokines in serum and intestine, enhancing the ability of superoxide dismutase to scavenge oxygen free radicals and increasing microcirculatory blood flow in acupoint area.
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