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| Molecular mechanism of Pingchuan Granules regulated EOS for asthmatic based on Notch/STAT signaling pathway |
| REN Yuzhe1 YU Zhou1 CHEN Hong2 YANG Zeyi3 YANG Xiangzheng |
1.Department of Pediatrics, Beijing University of Chinese Medicine, Guangdong Province, Shenzhen 518100, China;
2.Ward 1, Department of Pediatrics, the First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
3.School of Tradition Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China |
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Abstract Objective To investigate the therapeutic target and molecular mechanism of Pingchuan Granules in the treatment of asthma. Methods Eighty Wistar rats were randomly divided into Control group, Model group, Pingchuan Granule (PCG) group and Dexamethasone (DXMS) group, with 20 rats in each group. Asthma model rats were prepared by the method of combined inhalation of ovalbumin and aluminum hydroxide. Five days before the end of modeling, the corresponding drug intervention treatment was given by gavage. According to the modeling condition, ten of each group were selected for subsequent experiments. Bronchoalveolar lavage fluid (BALF) and serum eosinophilic granulocyte (EOS) counts, EOS cationic protein (ECP) and activated chemokine expression (Eotaxin) were compared among the four groups. Expression of proteins and mRNA related to Notch and STAT signaling pathways in lung were measured by RT-PCR and Western blot. Results BALF and serum EOS counts in Model group were higher than those in Control group (all P < 0.01), BALF and serum EOS counts in PCG group were lower than those in Model group (all P < 0.01). The content of ECP and Eotaxin in Model group were higher than those in Control group (all P < 0.01), and the content of ECP and eotaxin in PCG were lower than those in Model group (P < 0.05 or P < 0.01). Notch1, 2, 4, Hes, Jagged1, Delta4 protein and mRNA expression in Model group were higher than those in Control group, and the expression of Notch3, Delta1, 3 protein and mRNA were lower than those in Control group (all P < 0.01). Notch1, 2, 4, Hes, Jagged1, Delta4 protein and mRNA expression in PCG group were lower than those in Model group and the expression of Notch3, Delta1, 3 protein and mRNA were higher than those in Model group (P < 0.05 or P < 0.01). The expression of P-STAT1, 3, 5, 6 protein and mRNA in Model group were higher than those in Control group, and P-STAT4 protein and mRNA were lower than those in Control group (all P < 0.01). The expression P-STAT1, 3, 5, 6 protein and mRNA in PCG group were lower than those in Model group, and P-STAT4 protein and mRNA were higher than those in Model group (all P < 0.01). Conclusion PCG can reduce EOS by regulating Notch/STAT signaling pathway in the treatment of asthma.
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