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| Expression of TLR4 in maternal peripheral blood before and after parturition and its relationship with activation of downstream signaling pathways |
| CHEN Zixi1* ZHAO Yunzhi2* ZHU Zhaowei1 ZHANG Mengzhe1 LI Xiaoxiao1 XIANG Fenfen1 WU Rong1▲ KANG Xiangdong1▲ |
1.Department of Laboratory Medicine, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China;
2.Department of Obstetrics and Gynecology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China |
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Abstract Objective To analyze the expression of Toll-like receptor 4 (TLR4) in maternal peripheral blood before and after parturition and its relationship with activation of downstream signaling pathways. Methods The peripheral blood and uterine muscle tissue of pregnant women admitted to Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from March 2018 to August 2019 were collected and divided into term labor group (21 cases) and term non labor group (20 cases) according to whether or not labor. The expression of TLR4 was detected by flow cytometry and enzyme-linked immunosorbent assay. RT-PCR was used to detect the expression of TLR4, contraction related protein, nuclear factor (NF)-κB, JNK, ERK and P38 mRNA in uterine muscle, and their relationships were analyzed. Results There was no significant difference in age, gestational age and body mass index between two groups (P > 0.05). There were significant differences in the expression levels of peripheral blood neutrophils, monocytes and serum TLR4 between two groups (P < 0.05). There were significant differences in TLR4, CX43 and OTR between two groups (P < 0.05). There were significant differences in the mRNA levels of NF-κB, ERK, P38 and JNK between two groups (P < 0.05). TLR4 was positively correlated with CX43, OTR, JNK, ERK, P38 and NF-κB (R2 = 0.619, 0.416, 0.438, 0.426, 0.55, 0.332, all P < 0.01). JNK, ERK, P38 and NF-κB were positively correlated with CX43 (R2 = 0.548, 0.393, 0.702, 0.600, all P < 0.01), JNK, ERK, P38 and NF-κB were positively correlated with OTR (R2 = 0.308, 0.608, 0.532, 0.514, all P < 0.01). Conclusion TLR4 may play an important role in labor initiation by activating downstream NF-κB/MAPK pathway, promoting the expression of contraction related proteins and promoting uterine contraction.
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