冬凌草对代谢相关脂肪性肝病脂质代谢的影响

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Abstract Objective To investigate the effect of Rabdosia Rubescens on the expression of lipid metabolism-associated proteins in rats with metabolism-associated fatty liver disease (MAFLD). Methods The rats were fed with high-fat diet to replicate the metabolic-related fatty liver disease model. A total of 36 SD male rats were adaptively fed for one day, and they were divided into normal group, model group, and Rabdosia Rubescens low, medium, and high-dose groups by random number table method, each with six animals. The normal group was fed with ordinary feed, and the other groups were fed with high-fat feed. The low, medium, and high-doses of Rabdosia Rubescens were given 50, 100 mg/(kg·d) and 150 mg/(kg·d) per day, respectively; the normal group and the model group were given a gavage with the same volume of normal saline as the other experimental groups every day. HE staining was used to observe the degree of fatty degeneration of rat MAFLD liver tissue; automatic biochemical analyzer was used to detect triacylglycerol (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL- C), fasting blood glucose (FBG), fasting insulin (FINS); Western blot method was used to detect the expressions of peroxisome proliferator-activated receptor-α (PPAR-α), sterol regulatory element binding protein-1c (SREBP-1c), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC) protein. Results The liver tissue of rats in the normal group showed clear and complete structure under microscope, the structure of liver lobules was normal, and the hepatocyte cords were neatly arranged. Hepatocyte damage in the model group showed diffuse hepatocyte steatosis with balloon-like hepatocytes, inflammatory cell infiltration in the portal area, and partial punctate hepatocyte necrosis and detrital necrosis. The degree of liver damage in the Rabdosia Rubescens high-dose group was significantly less than that of the model group. The fatty degeneration of hepatocytes was reduced. There was only a few punctate necrosis, a few fat droplets, and no obvious inflammatory cell infiltration in the portal area. The levels of TC, TG, HDL-C, LDL-C, FBG, and FINS of rats in each group were compared, and the differences were statistically significant (P ＜ 0.05). The levels of serum TC, TG, LDL-C, FBG, and FINS in the model group were higher than those in the normal group, while the level of HDL-C was lower than that in the normal group, and the differences were statistically significant (P ＜ 0.05). The content of TG and FINS in the low-dose Rabdosia Rubescens group were lower than those of the model group, and the differences were statistically significant (P ＜ 0.05); the content of TC, TG, LDL-C, FBG, and FINS in the medium-dose Rabdosia Rubescens group were lower than those of the model group, while TG and FBG were lower than those of the low-dose Rabdosia Rubescens group, and the differences were statistically significant (P ＜ 0.05). The content of TC, TG, LDL-C, FBG, and FINS in the high-dose Rabdosia Rubescens group were lower than those of the model group, while the level of HDL-C was higher than that of the model group, and TC, TG, LDL-C, FBG were lower than those of the low-dose Rabdosia Rubescens group, and the differences were statistically significant (P ＜ 0.05). The expressions of PPAR-α and ACC protein in the model group were lower than those of the normal group, while the expressions of SREBP-1c and FAS protein were higher than those of the normal group, and the differences were statistically significant (P ＜ 0.05). The expressions of PPAR-α and ACC protein in the low dose of Rabdosia Rubescens group were higher than those of the model group, while the expression of FAS protein was lower than that of the model group, and the differences were statistically significant (P ＜ 0.05). The expressions of PPAR-α and ACC protein in the medium and high-doses Rabdosia Rubescens group were higher than those of the model group and the low-dose Rabdosia Rubescens group, while the expressions of SREBP-1c and FAS protein were lower than those of the model group and the low-dose Rabdosia Rubescens group, and the differences were statistically significant (P ＜ 0.05). Conclusion Rabdosia Rubescens can regulate lipid metabolism and associated protein expression in rats with MAFLD, which lays a foundation for further study on the mechanism of the occurrence and development of MAFLD.

Key words： Rabdosia Rubescens Metabolism-associated fatty liver disease Peroxisome proliferator-activated receptor-α Sterol regulatory element binding protein-1c Fatty acid synthase Acetyl-CoA carboxylase

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	MA Wenjun1 SHI Tingting2 BAI Minghui1 CHEN Xiaowei1 YANG Xingxin3▲

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MA Wenjun1 SHI Tingting2 BAI Minghui1 CHEN Xiaowei1 YANG Xingxin3▲. Effects of Rabdosia Rubescens on lipid metabolism in metabolism-associated fatty liver disease[J]. 中国医药导报, 2020, 17(25): 4-9.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I25/4

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