miR-203基因启动子区甲基化与肥胖膝关节骨性关节炎患者滑膜炎症水平相关性研究

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Abstract Objective To study the correlation between abnormal methylation of miR-203 gene promoter region in synovial tissues and the level of joint fluid proinflammatory factors in obese knee osteoarthritis (KOA). Methods Synovial tissue and joint fluid of KOA patients were taken from 36 patients who underwent knee arthroplasty in Handan Central Hospital in Hebei Province from January 2018 to January 2019. Patients with body mass index (BMI)≥28.0 kg/m2 were included in the obesity group (23 cases), and patients with 18.0 kg/m2≤BMI≤23.9 kg/m2 were included in the normal control group (13 cases). Methylation specific PCR (MSP) was used to detect the methylation status of the gene promoter region of miR-203 in synovial tissues. The expression level of miR-203 in synovial tissues was determined by quantitative polymerase chain reaction (qPCR). qPCR was used to detect the expression levels of miR-203 in the obese group and the normal control group. Pearson correlation analysis was used to investigate the correlation between miR-203 and BMI, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in joint fluid. Results In the synovial tissues of the obese group, the promoter region of miR-203 gene was abnormally hypomorphic, while in the normal control group, it was partially hypomorphic. Compared with the normal control group, the expression level of miR-203 in synovial tissues of the obesity group was increased, and the difference was statistically significant (P ＜ 0.05). The relative expressions of IL-1β, IL-6 and TNF-α in the joint fluid of the obese group were higher than those of the normal control group, with statistically significant differences (all P ＜ 0.05). The methylation level in the promoter region of miR-203 gene was negatively correlated with population BMI and the IL-1β, IL-6 and TNF-α in the joint fluid (all P ＜ 0.05). The expression level of miR-203 was positively correlated with population BMI and the IL-1β, IL-6 and TNF-α in the joint fluid (all P ＜ 0.05). Conclusion Hypophalation of miR-203 gene promoter in synovial tissues of KOA patients is closely related to BMI and levels of joint fluid proinflammatory factors in obese KOA patients, and miR-203 can be used as an important regulatory factor for inflammation of obese KOA and a potential target for treatment of KOA.

Key words： Obesity Knee osteoarthritis microRNA miR-203 Methylation

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	LI Jianwei1 ZHANG Li′na2 ZHANG Yingmin1 KANG Shaoying1 WEI Meng1

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LI Jianwei1 ZHANG Li′na2 ZHANG Yingmin1 KANG Shaoying1 WEI Meng1. Study on the correlation between the methylation of miR-203 gene promoter region and the level of synovitis in obese patients with knee osteoarthritis[J]. 中国医药导报, 2020, 17(12): 32-35,60.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I12/32

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