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| Study on the antibacterial, anti-inflammatory, antipyretic and antitussive effects of total flavonoids of Viola thianschanica Maxim. |
| WANG Xue SHI Yuzhu SI Lijun ZHAO Lijuan MA Xueping HUANG Hua |
| Xinjiang Institute of Materia Medica, Xinjiang Uygur Autonomous Region, Urumqi 830004, China |
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Abstract Objective To investigate the antibacterial, anti-inflammatory, antipyretic and antitussive effects of total flavonoids of Viola thianschanica Maxim.. Methods The doubling dilution method in vitro and establishment of mice infection model of Staphylococcus aureus, Escherichia coli in vivo were used to investigate the antibacterial effect of total flavonoids of Viola thianschanica Maxim. in vivo and in vitro. The model mice with auricle swelling and model mice with increased peritoneal capillary permeability were used to investigate the anti-inflammatory effects. The model rats with fever induced by dry yeast were adopted to investigate the antipyretic effect. The model rats with cough induced by ammonia water were adopted to investigate the antitussive effect. Results The minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of total flavonoids of Viola thianschanica Maxim. against Staphylococcus aureus were 1.95, 3.90 mg/mL respectively, and the MIC and MBC values of total flavonoids of Viola thianschanica Maxim. against Escherichia coli were 0.49, 0.98 mg/mL respectively. The total flavonoids of Viola thianschanica Maxim. had a significant protective effect on the infection model of Staphylococcus aureus and Escherichia coli in mice at 0.32 g/kg, the protective rates were 30% and 40% respectively. The total flavonoids of Viola thianschanica Maxim. could significantly inhibit auricle swelling induced by xylene in mice at 0.16, 0.32 g/kg, and could significantly inhibit capillary inflammatory exudation induced by glacial acetic acid in mice at 0.08, 0.16, 0.32 g/kg. The total flavonoids of Viola thianschanica Maxim. had a good inhibitory effect on fever induced by dry yeast in rats at 0.14, 0.28 g/kg. The total flavonoids of Viola thianschanica Maxim. had a significant inhibitory effect on cough induced by ammonia water, the inhibitory rate was 57.7% at 0.32 g/kg. Conclusion The total flavonoids extract of Viola thianschanica Maxim. has good antibacterial, anti-inflammatory, antipyretic and antitussive pharmacological activities, its mechanisms remain to be futher studied.
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