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| Pathological study of Baicalin combined with Berberine on two animal models of non-alcoholic steatohepatitis |
| XU Chang1 MA Zhijie2 HUANG Feng3 ZHONG Xiaosong1 |
1.Clinical Gene and Cell Engineering Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China;
2.Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;
3.Department of Acupuncture and Moxibustion, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China |
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Abstract Objective To investigate the effect of Baicalin combined with Berberine on non-alcoholic steatohepatitis (NASH) in two animal models from the pathological point of view. Methods 40 SD rats and 40 C57BL/6 mice aged 6 weeks were divided into four groups according to random number table method: normal control group, NASH model group, Berberine group, Baicalin + Berberine group, 10 rats (or mice) in each group. In addition to normal control group, the other three groups prepared SD rats NASH model induced by high fat diet and C57BL/6 mice NASH model induced by methionine-choline deficiency diet. Berberine group was given Berberine by gavage, Baicalin + Berberine group was given Baicalin and Berberine by gavage. The rats were taken after 8 weeks, and the mice were taken after 2 weeks. Paraffin sections of liver tissue were stained with HE, frozen sections were stained with oil red O. After light microscopy, non-alcoholic fatty liver disease activity scale (NAS) was used to evaluate NASH in each group. Results The scores of hepatic steatosis, intralobular inflammation, hepatocyte balloon-like degeneration and total NAS in NASH model group were significantly higher than those in normal control group of two animal models (P < 0.01). The scores of hepatic steatosis, intralobular inflammation, hepatocyte balloon-like degeneration and NAS in Berberine group and Baicalin + Berberine group were significantly lower than those in NASH model group of two animal models (P < 0.05 or P < 0.01). Conclusion Baicalin and Berberine can significantly inhibit the occurrence and development of pathological changes in NASH. They have certain intervention effect on NASH.
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