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| Expression of polar protein Par3 in cervical squamous cell carcinoma and its clinical significance |
| GUO Chunfeng ZHAO Junda MA Junqi |
| Department of Gynecology, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China |
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Abstract Objective To explore the polarity protein Par3 (Par3 protein) in chronic cervicitis, cervical intraepithelial neoplasia Ⅱ-Ⅲ (CINⅡ-Ⅲ), cervical squamous cell carcinoma (CSCC) and the expression differences in metastasis lymph node tissue and its clinical significance. Methods From January 2016 to December 2017, Xinjiang Medical University First Affiliated Hospital of CINⅡ-Ⅲ CSCC, as well as the line of hysterectomy for benign disease patients with cervical tissue samples archived paraffin embedding tissue, a total of 93 cases were seleeted from Department of Gynaecology. Among them 14 cases of chronic cervicitis, 36 cases of CINⅡ-Ⅲ, and 43 cases of cervical squamous cell carcinoma. Another 7 cases of cervical squamous cell carcinoma lymph node metastasis tissue samples. The expression of polar protein Par3 in the above tissues was detected by immunohistochemical staining. Results The positive expression rate of Par3 protein in chronic cervicitis, CINⅡ-Ⅲ, CSCC and metastasis lymph node tissues were 100.0% (14/14), 97.2% (35/36), 62.8% (27/43), 0 respectively, the differences between each group had statistical significance (P ＜ 0.05). The Par3 protein expression was 100% (7/7) in the high differentiation group of CSCC, while the expression was down-regulated to 55.6% (20/36) in the low and medium differentiation group, with statistically significant difference (P ＜ 0.05). They were grouped according to whether lymph node metastasis had occurred (7 cases in lymph node metastasis group 36 cases in the group without lymph node metastasis. The positive expression rate of Par3 protein in cervical tissues of the lymph node metastasis group was 28.6% (2/7), while that of the non-metastasis group was 58.3% (21/36), with statistically significant difference (P ＜ 0.05). The low expression of Par3 protein was correlated with histological differentiation and lymph node metastasis, and the difference was statistically significant (P ＜ 0.05). Conclusion Polarity protein Par3 in chronic cervicitis, CINⅡ-Ⅲ CSCC and are differentially expressed, the expression of CSCC is down-regulated, and its abnormal low expression is related to the occurrence and development of CSCC, which can be used as an early warning indicator to judge the development of cervical intraepithelial neoplasia into CSCC, as well as an indicator to judge the malignancy degree and prognosis of CSCC.
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