低氧预处理人胎盘绒毛膜间充质干细胞环状RNA筛选

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Abstract Objective To analyze the expression profile variation of circular RNA (circRNA) between hypoxia preconditioned human placenta chorionic mesenchymal stem cells (H-hpcMSCs) and normoxic preconditioned hpcMSCs (N-hpcMSCs). Methods Human circRNA microarray was performed to detect the difference of circRNA expression between 3 paired specimens of H-hpcMSCs and its N-hpcMSCs. The circRNA differental expression and the predicted miRNA of differentially expressed circRNA were analyzed. Results Of the 13 617 circRNAs, 12 114 circRNAs with analytical results. In H-hpcMSCs, a total of 102 circRNAs showed differentially expressed which have more than 1.5 times variation and significant difference (P < 0.05). Among them, 85 increased and change′s fold more than 1.5 times, while 17 reduced and change′s fold more than 1.5 times, a total of 27 circRNAs showed a greater than 2-fold change and all were up-regulated. Five miRNAs of each with differentially expressed circRNA were predicted according to specific base pairing. Conclusion The circRNA expression profile of H-hpcMSCs changed significantly in comparison with N-hpcMSCs, the circRNA may be associated with the hypoxia precondition.

Key words： Hypoxia precondition Human placenta chorionic mesenchymal stem cells Circular RNA Expression profile

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	Articles by authors
	SUN Xunsha1 WU Jieying2
	3 CHEN Jinsong2
	3 LU Yan2
	3 YU Qiuxia2
	3 LI Ru2
	3 WANG Dan2
	3 LI Yan2
	3 WU Shaoqing2
	3▲

Cite this article:

SUN Xunsha1 WU Jieying2,3 CHEN Jinsong2,3 LU Yan2, et al. Differentially expression profile of circular RNAs in hypoxia-preconditioned human placenta chorionic mesenchymal stem cells[J]. 中国医药导报, 2018, 15(18): 9-11.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I18/9

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