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| The behavior study on the increase of susceptibility to epilepsy in Wistar rats by β-amyloid protein |
| ZHAO Juan1 ZHANG Yanmei2 ZHENG Naizhi1 |
1.Department of Neurology, Qingdao Municipal Hospital Affiliated to Qingdao University, Shandong Province, Qingdao 266011, China;
2.Department of Primay Medical Management, Qingdao Municipal Hospital Affiliated to Qingdao University, Shandong Province, Qingdao 266011, China |
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Abstract Objective To study the effects of β-amyloid protein (Aβ) on the pathogenesis of epilepsy (EP). Methods 40 male Wistar rats were divided into Aβ1-42 + PILO group, Aβ1-42 group, PBS + PILO group and PBS group by random number table, with 10 rats in each group. The former two groups were called Aβ1-42 model group and the later PBS control group. Aβ1-42 and same volume PBS were injected into the hippocampal CA3 region of Wistar rats in Aβ1-42 model group and PBS control group by stereotaxic apparatus respectively, and the Morris water maze test was observed after 2 weeks. Escape latency, the percentage of swimming time in the target quadrant and the number of times of crossing the platform were recorded. After the AD model was successfully made, the rat model of chronic EP was established by intraperitoneal injection of LI-PILO. The latency of EP, degree of seizure and mortality of rats were recorded according to Racine grading criteria. Results One rat died in Aβ1-42 model group and the PBS control group. The results of Morris water maze test showed that compared with the PBS control group, the escape latency of the Aβ1-42 model group was longer (P < 0.01), the proportion of swimming time in the target quadrant was shorter (P < 0.05), and the number of crossing the platform was also significantly reduced (P < 0.05). It indicated that the AD model was successfully prepared. Preparation of rats EP model: The latency of EP in Aβ1-42 + PILO group was significantly shorter (P < 0.01), and the degree of seizure was more severe (P < 0.05), compared with PBS + PILO group, according to Racine grading criteria. The mortality of rats in Aβ1-42 + PILO group was significantly increased compared with PBS + PILO group (P < 0.05). Conclusion It can lead to the impaired hippocampus-related learning and memory function by injection of Aβ1-42 in hippocampal CA3 region of rats. The existing of Aβ increasing the susceptibility to EP in the rats, which indicates that Aβ plays a facilitation role in the occurrence of EP.
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