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| Effects of hypoxia on mechanism of TH17 in spinal tuberculosis recurrence |
| Suonan′angxiu CAO Zhenyu QI Tengmin ZHOU Jingyuan LI Ming AN Yuxiu TANG Weidong HAN Guodong |
| Department of Orthopedics, Qinghai People's Hospital, Qinghai Province, Xining 810007, China |
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Abstract Objective To discuss the effect of TH17 cells and cytokines on the recurrence of spinal tuberculosis was evaluated in hypoxic environment. Methods From September 2015 to January 2017, in Qinghai People's Hospital, 46 patients with spinal tuberculosis were selected, they were divided into primary group (27 cases) and recurrence group (19 cases) according to clinical manifestation and laboratory examination. TH17 cells and cytokines including IL-6, IL-1β, IL-23, TNF-α, IFN-γ in patients and the CCL20, CCL22, CCL27 were detected. The relationship of chemotactic index of Th17 cells and the Th17 expression of CCR receptor and CCL ligand were evaluated. Results Compared with primary group, the percentage of TH17 cells increased significantly of patients in recurrence group (P < 0.05), the content of cytokines including IL-6, IL-1, IL-23, TNF-α increased significantly (P < 0.01), the content of IFN-γ decreased (P < 0.01). The concentration of CCL20, CCL22 and CCL27 from recurrent group were significantly higher than primary group (P < 0.01), immunofluorescence staining and scanning quantitative analysis showed that the expression of CCL20, CCL22 and CCL27 in bone cells of patients in recurrent group were higher than in primary group (P < 0.01), cell morphology was similar with the the staining results of primary group. Conclusion Th17 cells can be used for the diagnosis of tuberculosis and tuberculosis recurrence, differentiation of tuberculosis courses and evaluation of immune function of patients. IL-6, IL-1β, IL-23, TNF-α, IFN-γ, CCL20, CCL22, CCL27, CCR receptors and CCL ligands can be used as a new diagnostic marker for the recurrence of spinal tuberculosis in hypoxia, and for the early diagnosis of tuberculosis recurrence.
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