|
|
|
| Comparison of the consistency of blood ALT test results inside and outside station and analysis of the cause of the error |
| ZHAO Jianpeng1 YANG Xiaoya2▲ YANG Xiuping3 XU Yunpeng1 LI Lihong4 |
1.Zhangjiakou Central Blood Station, Hebei Province, Zhangjiakou 075000, China;
2.Dezhou United Hospital, Shandong Province, Dezhou 253000, China;
3.Zhangjiakou Maternal and Child Health Care Hospital, Hebei Province, Zhangjiakou 075000, China;
4.College of Medical Laboratory, Hebei North University, Hebei Province, Zhangjiakou 075000, China |
|
|
|
Abstract Objective To compare the detection results of alanine aminotransferase (ALT) test of blood donors inside and outside the station, in order to develop corresponding measures to ensure the consistency of the results of different instruments and equipments. Methods The same donors′ blood ALT were measured by automatic biochemical analyzer Hitachi 7100 and semi-automatic biochemical analyzer Photometer 4040 respectively. The results obtained by the Hitachi 7100 were taken as standard results X, and the results of Photometer 4040 were takend as unevaluated results Y (the results of Photometer 4040 on the donation car were Y1; the results of Photometer 4040 in the station were Y2). The dn was calculated by Yn subtracting X, the differences between d1 and d2 were compared, and line charts of dn were made to directly reflect differences in ALT testing results outside and inside the station. The factors leading to different results were analyzed. Results Difference value d1 of Hitachi 7100 and outside blood station 4040 test results was (5.07±4.33), difference value d2 of Hitachi 7100 and inside blood station 4040 test results was (2.40±1.39), the difference was significant (t=7.513, P < 0.05). The fluctuation range of the d1 line chart was obviously higher than that of d2 line chart. Conclusion The instability of the ALT results obtained by semiautomatic biochemical analyzer outside the station is significantly higher than that inside the station. There are many reasons for this result, such as environmental factors of experimental operation, the stability of electric current and voltage of instrument and hemolysis as well as other blood problems in blood collection process.
|
|
|
|
|
|
[1] 吴君胜，张胜权.合肥地区无偿献血者在献血前进行ALT快速检测的现状及意义[J].安徽医药，2012，16（3）：363-364.
[2] Sohn W，Jun DW，Kwak MJ，et al. Upper limit of normal serum alanine and aspartate aminotransferase levals in Korea [J]. J Gastroenterol Hepatol，2013，28（3）：522-529.
[3] Prati D，Taioli E，Zanella A，et al. Updated definitions of healthy ranges for serum alanine aminotransferase levels [J]. Ann lntern Med，2002，137（1）：1-10.
[4] 刘萍丽，张峰波，朱有森.乌鲁木齐市汉族健康人群丙氨酸氨基转移酶及天门冬氨酸氨基转移酶参考区间的初步调查[J].国际检验医学杂志，2013，34（7）：808-810.
[5] 左志平，王智红，孙国栋，等.ALT单项不合格献血者献血情况跟踪调查分析[J].国际检验医学杂志，2014，35（17）：2326-2327.
[6] 李聚林，许建荣，覃益康，等.献血前ALT干式化学法初筛不合格者回归献血情况分析[J].中国输血杂志，2014， 27（11）：1219-1221.
[7] 何子毅，邹文涛，王德文，等.ALT单项检测不合格献血者追踪调查分析[J].中国输血杂志，2009，22（4）：296-298.
[8] 李天君，赵锋，张文学，等.采供血机构不同检测系统对ALT 检测结果比对研究[J].中国输血杂志，2012，25（9）：849-851.
[9] 王智红，尹志柱，孙国栋，等.街头初筛ALT对献血者及采供血量的影响[J].国际检验医学杂志，2014，35（3）：365-367.
[10] 掌友湖，梁启忠，程玉根，等.ALT干式化学法与全自动速率法检测的对比试验[J].中国输血杂志，2013，26（8）：721-722.
[11] 侯广安，高占海，张媛，等.流动采血车与检验科ALT检测结果比对及影响因素分析[J].西部医学，2014，26（4）：518-522.
[12] 邹文涛，王铁兵，何子毅，等.无偿献血者ALT报废域值与NAT-IBV/HCV检测结果分析[J].中国输血杂志，2008，21（12）：930-932
[13] 中华人民共和国卫生和计划生育委员会.《血站操作技术规程（2015版）》[S].国卫医发〔2015〕95号附件.2016-03-01.
[14] 张秀明，庄俊华，徐宁，等.不同检测系统血清酶测定结果的偏倚评估与可比性研[J].中华检验医学杂志，2006， 29（4）：346-349.
[15] 季阳，王迅，郑忠伟，等.重新评估献血者ALT检测的意义[J].中国输血杂志，2009，22（7）：521-522.
[16] 张维.运用设各比对及改进初筛设各降低血液ALT报废率[J].职业与健康，2010，26（11）：1225-1226.
[17] Notari EP，Orton SL，Cable RG，et al. Seroprevalence of known and putative hepatitis markers in United States blood donors with ALT levels at least 120 IU per L [J]. Transfusion，2001，41（6）：751-755.
[18] 侯广安，高占海，张媛，等.流动采血车与检验科ALT检测结果比对及影响因素分析[J].西部医学，2014，26（4）：518，522.
[19] 李红.静脉血与末梢血检验结果的对比分析[J].中国保健营养：下半月，2012（14）：561.
[20] 葛红卫，王鸿捷，沈莉，等.丙氨酸氨基转移酶异常血液报废原因分析[J].中国输血杂志，2001，24（14）：977-978. |
|
|
|
