黄芪多糖抑制糖尿病心肌氧化应激的初步研究

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摘要目的探讨黄芪多糖对糖尿病心肌氧化应激的影响。方法将16只6～8周龄的雄性健康转基因SOD2+/-KO小鼠（C57BL/6J背景）按照随机数字表法随机分为转基因组及黄芪多糖转基因组（n = 8）；将24只正常对照小鼠分为对照组、糖尿病组及黄芪多糖糖尿病组（n = 8）。糖尿病组和黄芪多糖糖尿病组采用链脲佐菌素腹腔注射法制作糖尿病动物模型，糖尿病模型建成功后，给予黄芪多糖糖尿病组和黄芪多糖转基因组小鼠黄芪多糖注射液治疗16周，其余组则给予等量生理盐水灌胃。采用超声心动图检测心脏功能，HE染色观察心肌组织结构，超声电镜观察心肌超微结构，免疫组化法检测心肌细胞增殖活性，TUNEL法检测心肌细胞凋亡，荧光定量法检测心肌活性氧（ROS）含量，Western blot法检测心肌SOD2蛋白含量，SODⅡ试剂盒检测心肌SOD2酶活性。结果与对照组比较，糖尿病组和转基因组LVSP、±LVdp/dt下降，LVEDP升高，心肌组织和超微结构存在严重损伤，细胞凋亡和坏死程度增加，SOD2活性被显著抑制，而黄芪多糖治疗组小鼠以上指标均得到改善（P ＜ 0.05）。结论黄芪多糖能抑制糖尿病心肌的氧化应激损伤。

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	陈蔚1▲ 鞠婧1 王浩2 王莉英2 杨叶虹3 叶红英3 李益明3 张玉1▲

关键词 ：黄芪多糖, 糖尿病, 心肌病, 氧化应激, SOD2

Abstract：Objective To investigate the effect of Astragalus polysaccharides for myocardial oxidative stress of diabetes mellitus. Methods Sixteen male healthy transgenic SOD2+/-KO mice aged 6-8 weeks old (C57BL/6J) were randomly divided into transgenic group and Astragalus polysaccharides transgenic group by random number table method (n = 8). The 24 normal control mice were divided into control group, diabetes mellitus group and Astragalus polysaccharides diabetes mellitus group (n = 8). Single intraperitoneal injection of Streptozotocin was utilized to establish mice model with diabetes mellitus in diabetes mellitus group and Astragalus polysaccharides diabetes mellitus group, after the diabetes mellitus model was made successfully, the mice in Astragalus polysaccharides diabetes mellitus group and Astragalus polysaccharides transgenic group were treated with Astragalus Polysaccharides Injection for 16 weeks, and the other groups were given equal amount of normal saline. Echocardiography was used to detect cardiac function, HE staining was used to observe the structure of myocardial tissue, ultrasonic electron microscopy was used to observe the myocardial ultrastructure, immunohistochemistry was used to detect the proliferative activity of cardiomyocytes, TUNEL method was used to detect apoptosis of cardiomyocytes; fluorescent quantitative assay was used to detect the contents of myocardial reactive oxygen species (ROS), Western blot was used to detect the contents of myocardial SOD2 protein, calbiochem SOD assay kit Ⅱ was used to detect the activity of myocardial SOD2. Results Compared with the control group, LVSP and ±LVdp/dt in diabetes mellitus group and transgenic group were decreased, and LVEDP was increased, myocardial tissue and ultrastructure were seriously damaged, the degree of cells apoptosis and necrosis was increased, and the activity of SOD2 was significantly inhibited. However, the indices above in the Astragalus polysaccharides were all signifcantly improved (P ＜ 0.05). Conclusion Astragalus polysaccharides can inhibit the myocardial oxidative stress injury of diabetes mellitus.

Key words： Astragalus polysaccharide Diabetes Cardiomyopathy Oxidative stress SOD2

基金资助:国家自然科学基金面上项目（81473460）。

通讯作者: ▲共同通讯作者

引用本文:

陈蔚1▲ 鞠婧1 王浩2 王莉英2 杨叶虹3 叶红英3 李益明3 张玉1▲. 黄芪多糖抑制糖尿病心肌氧化应激的初步研究[J]. 中国医药导报, 2018, 15(9): 4-7.
CHEN Wei1▲ JU Jing1 WANG Hao2 WANG Liying2 YANG Yehong3 YE Hongying3 LI Yiming3 ZHANG Yu1▲. Primary study of Astragalus polysaccharides in inhibiting myocardial oxidative stress of diabetes mellitus . 中国医药导报, 2018, 15(9): 4-7.

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