Construction and identification of a recombinant lentivirus with overexpression Slug gene
ZHOU Yuanhong1 LUO Youzhen1 ZHOU Xiaolin2 LIU Qiang2
1.Department of Gynecology, the First College of Clinical Medical Science, Three Gorges University, Hubei Province, Yichang 443000, China;
2.Department of Infectious Disease, the First College of Clinical Medical Science, Three Gorges University, Hubei Province， Yichang 443000, China
Abstract：Objective To construct recombinant lentivirus with Slug gene for further exploring the effect of Slug in metastatic recurrence. Methods The Slug gene was acquired from the breast cancer cell MDA-MB-231 by PCR assay, which was then connected into linearized GV367 vector digested by Age Ⅰ/Nhe I enzyme. The positive clones were identified by PCR and sequencing analysis. The constructed lentiviral vectors with Slug gene were then transfered into 293T cell lines. The lentivirus titer was detected by the fluorescence method. Results The lentiviral vector with Slug gene was successfully constructed and obtained, the virus titer of which was 5×108 TU/mL. Conclusion The lentiviral with Slug gene are successfully constructed, which will provide the experimental foundation for further exploring the effect of Slug in metastatic recurrence.
周园红1 罗幼珍1 周晓琳2 刘强2. 人转录因子Slug过表达慢病毒载体的构建与鉴定[J]. 中国医药导报, 2018, 15(7): 12-15.
ZHOU Yuanhong1 LUO Youzhen1 ZHOU Xiaolin2 LIU Qiang2. Construction and identification of a recombinant lentivirus with overexpression Slug gene. 中国医药导报, 2018, 15(7): 12-15.
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2018, Vol. 15 
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