Effect of Zhuanggu Zhitong prescription on CGRP and β2-AR in ovariectomized osteoporosis rats
GAN Guoxing1 LIU Yu1 LI Jinping2 ZHANG Guomin3 YI Zhongyuan1
1.Ministry of Science and Education, Qingyuan Hospital of Traditional Chinese Medicine, Guangdong Province, Qingyuan 511500, China;
2.Xiangya School of Pharmacy, Central South University, Hu′nan Province, Changsha 410013, China;
3. Basic Medical College, Hunan University of Traditional Chinese Medicine, Hu′nan Province, Changsha 410028, China
Abstract：Objective To explore the effect of Zhuanggu Zhitong prescription on the CGRP and β2-AR in ovariectomized osteoporosis rats and investigate its mechanism in anti-osteoporosis. Method 72 rats were randomly divided into sham operation group (12 cases) and model group (60 cases), the model group rats were ovariectomized osteoporosis and the sham operation group rats were excised equal weight fat. After the modeling, the rats were randomly divided into model group, Zhuanggu Zhitong prescription high-dose group (13.2 g/kg), middle-dose group (6.6 g/kg), low-dose group (3.3 g/kg) and estradiol valerate group (2.1×10-5 g/kg), with 12 rats in each group. The model group and sham operation group rats were given equal volume water. All intervened for 13 weeks. The content of CGRP in serum was detected by ELISA and protein expression of β2-AR in bone tissue were detected by immunohistochemistry. Results Compared with sham operation group, the content of CGRP in serum and protein expression of β2-AR in bone tissue of model group were significantly declined, with high statistically significant differences (all P ＜ 0.01). Compared with the model group, the content of CGRP in serum and protein expression of β2-AR in bone tissue of different doses Zhuanggu Zhitong prescription group and estradiol valerate group were increased significantly, with statistically significant differences (P ＜ 0.05 or P ＜ 0.01). There were no significant differences in different doses of Zhuanggu Zhitong prescription groups (P ＞ 0.05). Conclusion Zhuanggu Zhitong prescription can promote the secretion of CGRP and protein expression of β2-AR, which may be one mechanism of ZhuangguZhitong prescription to anti-osteoporosis.
甘国兴1 刘毓1 李劲平2 张国民3 易仲媛1. 壮骨止痛方对去卵巢骨质疏松大鼠CGRP及β2-AR的影响[J]. 中国医药导报, 2018, 15(7): 9-11,15.
GAN Guoxing1 LIU Yu1 LI Jinping2 ZHANG Guomin3 YI Zhongyuan1. Effect of Zhuanggu Zhitong prescription on CGRP and β2-AR in ovariectomized osteoporosis rats. 中国医药导报, 2018, 15(7): 9-11,15.
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2018, Vol. 15 
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