血小板miR-96-5p在血小板储存过程中抗凋亡机制的研究

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摘要目的研究血小板微RNA（microRNA）基因miR-96-5p在血小板储存过程中抗凋亡的机制，探讨血小板储存时间的影响因素。方法用定量PCR方法检测储存起始和储存至第5天的健康血液捐献者的单采血小板中的24种与凋亡相关的miRNAs的表达水平的变化，检测结果进行统计学分析，判断miRNA在调节血小板存活或者凋亡方面的作用。结果与储存起始比较，血小板储存至第5天miR-96-5p、miR-16-5p、miR-155-5p、miR-148a-5p和miR-296-5p的表达显著上升（P < 0.01），而miR-7-5p、miR-145-5p、miR-15a-5p、miR-25-3p以及miR-24-3p的表达显著下降（P < 0.01）。转染miR-96-5p抑制物至血小板72 h后，增加了细胞半胱氨酸蛋白酶-3（caspase-3）的活性，抑制了Bcl-2和Bcl-xl的表达，促进了Bax的积累和活性caspase-3的加工。结论 miR-96-5p在血小板储存中可能发挥抗凋亡作用。

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	徐恒仕1 杨英1 崔恒祥2

关键词 ： microRNA, miR-96-5p, 血小板, 抗凋亡, caspase-3

Abstract： Objective To study the anti-apoptosis mechanism of miR-96-5p in platelet during the storage, and explore the factor affecting the platelet storage. Methods The expression changes of apoptosis related 24 miRNAs in apheresis platelets on the starting and 5 days of the storage was detected by quantitative real time PCR, and the results were statistically analyzed. The effect of miRNAs on survival and apoptosis of platelet. Results Compared with the starting of the storage, the expression of miR-96-5p, miR-16-5p, miR-155-5p, miR-148a-5p and miR-296-5p on 5 days of the platelet storage was significantly increased (P < 0.01), while the expression of miR-7-5p, miR-145-5p, miR-15a-5p, miR-25-3p and miR-24-3p was significantly decreased (P < 0.01). 72 h after transfected the miR-96-5p inhibitors into the platelet, the activity of caspase-3 was increased and the expression of Bcl-2 and Bcl-xl proteins was decreased, the process of Bax accumulation and active of caspase-3 was promoted. Conclusion miR-96-5p may play a role in the anti-apoptosis of platele storage.

Key words： microRNA miR-96-5p Platelet Anti-apoptosis caspase-3

基金资助: 上海市第四轮公共卫生重点学科建设项目（15GWZK0501）。

通讯作者: 杨英（1973.5-），女，主管检验师，主要从事临床输血工作。

引用本文:

徐恒仕1 杨英1 崔恒祥2. 血小板miR-96-5p在血小板储存过程中抗凋亡机制的研究[J]. 中国医药导报, 2018, 15(4): 13-17.
XU Hengshi1 YANG Ying1 CUI Hengxiang2. Study of the mechanism of miR-96-5p in anti-apoptosis induced by storage in platelet . 中国医药导报, 2018, 15(4): 13-17.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2018/V15/I4/13

[1] Mason KD，Carpinelli MR，Fletcher JI，et al. Programmed anuclear cell death delimits platelet life span [J]. Cell，2007，128（6）：1173-1186.
[2] Zhang H，Nimmer PM，Tahir SK，et al. Bcl-2 family proteins are essential for platelet survival [J]. Cell Death Differ，2007，14（5）：943-951.
[3] Perales JP，Figueredo R，Guan Y，et al. Increased platelet storage time is associated with mitochondrial dysfunction and impaired platelet function [J]. J Surg Res，2013，184（1）：422-429.
[4] Gyulkhandanyan AV，Mutlu A，Freedman J，et al. Markers of platelet apoptosis：methodology and applications [J]. J Thromb Thrombolysis，2012，33（4）：397-411.
[5] Willeit P，Zampetaki A，Dudek K，et al. Circulating microRNAs as novel biomarkers for platelet activation [J]. Circ Res，2013，112（4）：595-600.
[6] McManus DD，Freedman JE. MicroRNAs in platelet function and cardiovascular disease [J]. Nat Rev Cardiol，2015， 12（12）：711-717.
[7] Kannan M，Mohan KV，Kulkarni S，et al. Membrane array-based differential profiling of platelets during storage for 52 miRNAs associated with apoptosis [J]. Transfusion，2009，49（7）：1443-1450.
[8] Yu S，Deng G，Qian D，et al. Detection of apoptosis-associated microRNA in human apheresis platelets during storage by quantitative real-time polymerase chain reaction analysis [J]. Blood Transfus，2014，12（4）：541-547.
[9] 杜红俊，惠延年，王雨生，等.Caspase-3在柔红霉素诱导的人视网膜色素上皮细胞凋亡中作用[J].第四军医大学学报，2001，22（7）：609-611.
[10] Vishwamitra D，Li Y，Wilson D，et al. MicroRNA 96 is a post-transcriptional suppressor of anaplastic lymphoma kinase expression [J]. Am J Pathol，2012，180（5）：1772-1780.
[11] Yamada Y，Enokida H，Kojima S，et al. MiR-96 and miR-183 detection in urine serve as potential tumor markers of urothelial carcinoma：correlation with stage and grade，and comparison with urinary cytology [J]. Cancer Sci，2011，102（3）：522-529.
[12] Yoshino H，Seki N，Itesako T，et al. Aberrant expression of microRNAs in bladder cancer [J]. Nat Rev Urol，2013， 10（7）：396-404.
[13] Yan Z，Wang J，Wang C，et al. miR-96/HBP1/Wnt/beta-catenin regulatory circuitry promotes glioma growth [J]. FEBS Lett，2014，588（17）：3038-3046.
[14] Feng J，Yu J，Pan X，et al. HERG1 functions as an oncogene in pancreatic cancer and is downregulated by miR-96 [J]. Oncotarget，2014，5（14）：5832-5844.
[15] Guttilla IK，White BA. Coordinate regulation of FOXO1 by miR-27a，miR-96，and miR-182 in breast cancer cells [J]. J Biol Chem，2009，284（35）：23 204-23 216.
[16] Haflidadottir BS，Larne O，Martin M，et al. Upregulation of miR-96 enhances cellular proliferation of prostate cancer cells through FOXO1 [J]. PLoS One，2013，8（8）：e72 400.