Abstract:Objective To improve the quality standard of Huangdi Jixian Tablets, high performance liquid chromatography (HPLC) method was established to detect the contents of hyperin and the contents of heavy metal and arsenic salt in Huangdi Jiexian Tablets were detected. Methods Symmetry C18 (5 μm, 6 mm×150 mm) column was used. The mobile phase contained acetonitrile and 0.1% phosphoric acid (18∶82). The flow rate was 1.0 mL/min and the detection wavelength was 353 nm. The injection volume was 10 μL. In addition, the impurity of heavy metal and arsenic salt was also detected according to Chinese Pharmacopoeia (2010). Results Within the range from 2.0 to 100.0 μg/mL, hyperin presented an ideal linear relationship with the peak areas (R2 = 0.9998). The average recovery rate was 75.61%. Heavy metal was less than 10 parts per million and arsenic salt was less than 2 parts per million. These results conformed to the requirements of Chinese Pharmacopoeia (2010). Conclusion HPLC method for determining the content of hyperin is simple, with high sensitivity and good repeatability, which can be used for the content determination of hyperin in Huangdi Jixian Tablets. The contents of heavy metal and arsenic salt are conformed to the requirement, which provides evidence for the safety of clinical medication.
田蜜 陈芳 贺建昌. 黄地吉仙片中金丝桃苷含量测定方法的建立[J]. 中国医药导报, 2018, 15(3): 122-125.
TIAN Mi CHEN Fang HE Jianchang. Establishment of method for determination of the contents of hyperin in Huangdi Jixian Tablets. 中国医药导报, 2018, 15(3): 122-125.