Abstract：Objective To establish a high performance liquid chromatography-mass spectrometry / mass spectrometry (LC-MS/MS) method for the determination of seven quinolones residues in Thyroid Tablets. Methods The column was ACQUITY UPLC?誖BEH C18 (2.1 mm×100 mm, 1.7 μm). Acetonitrile was used as mobile phase A and 0.1% formic acid as mobile phase B for gradient elution. The flow rate was 0.3 mL/min, the column temperature was 40℃. LC-MS/MS method, electrospray ionization source, multiple reaction monitoring and positive ion scanning method were used for detection. Results Seven quinolones showed good linear relationship in the concentration range of 1-100 ng/mL. The average recoveries of seven kinds of quinolones were in the range of 85.0%-100.0%, and RSDs were all less than 3.5%. Conclusion The established method is sensitive, accurate, stable, with little interference from impurities and strong specificity. It can be used for the determination of quinolone residues in Thyroid Tablets.
2021, Vol. 18 
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