Effects of overexpression of galectin-9 protein on proliferation, migration and apoptosis of ovarian cancer cells
CHENG Yayu1 TAN Xueying2 HUANG Fei2 ZHANG Ping3
1.College of Clinical Medicine, Weifang Medical University, Shandong Province, Weifang 261042, China;
2.Department of Hepatobiliary Surgery, Qingdao Municipal Hospital Affiliated to Medical College of Qingdao University, Shandong Province, Qingdao 266000, China;
3.Department of Gynecology, Qingdao Municipal Hospital Affiliated to Medical College of Qingdao University, Shandong Province, Qingdao 266000, China
Abstract：Objective To investigate the effects of overexpression of galectin-9 on the proliferation, migration and apoptosis of ovarian cancer cells. Methods The ovarian cancer cells SKOV-3 were divided into pcDNA3.1-galectin-9 transfection group, pcDNA3.1 group and blank control group, Western blot was used to detect the expression of galectin-9 protein, CCK-8 was used to detect the effects of galectin-9 protein for multiplication capacity of SKOV-3, scratch test was used to verify the effects of galectin-9 protein for transfer ability of SKOV-3, Annexin-V-FITC/PI double staining method was used to detect the effects of galectin-9 protein for apoptosis of SKOV-3. Results The results of Western blot showed that the expression of galectin-9 protein in pcDNA3.1-galectin-9 transfection group was significantly higher than those in blank control group and pcDNA3.1 group, the differences were all highly statistically significant (all P ＜ 0.01). The results of CCK-8 showed that the multiplication capacity of galectin-9 in pcDNA3.1-galectin-9 transfection group was significantly lower than those in blank control group and pcDNA3.1 group, the differences were all highly statistically significant (all P ＜ 0.01), galectin-9 could significantly inhibit the multiplication capacity of cells, and the capacity of inhibiting proliferation would increase with the extension of time. The results of cell scratch assay showed that the healing rate of pcDNA3.1-galectin-9 transfection group was significantly higher than that of pcDNA3.1 group and blank control group, the differences were all highly statistically significant (all P ＜ 0.01). The results of flow cytometry showed that the apoptosis rate of pcDNA3.1-galectin-9 transfection group was significantly higher than that of pcDNA3.1 group and blank control group, the differences were highly statistically significant (all P ＜ 0.01). Conclusion The overexpression of galectin-9 can inhibit the proliferation and migration of ovarian cancer cell lines and promote its apoptosis.
程亚玉1 谭雪莹2 黄飞2 张萍3 . Galectin-9蛋白过表达对卵巢癌细胞增殖、迁移及凋亡的影响[J]. 中国医药导报, 2018, 15(3): 13-16.
CHENG Yayu1 TAN Xueying2 HUANG Fei2 ZHANG Ping3. Effects of overexpression of galectin-9 protein on proliferation, migration and apoptosis of ovarian cancer cells. 中国医药导报, 2018, 15(3): 13-16.
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2018, Vol. 15 
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