百里醌对小鼠辐射防护作用

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摘要目的探讨百里醌对辐射小鼠损伤的防护作用。方法 C57BL/6J小鼠按体重随机分为3组（n=5）：空白对照组、单纯照射组和照射给药组。其中单纯照射组和照射给药组给予7.5 Gy全身照射，空白对照组给予假照射。照射给药组在照射前1 d灌胃给药（10 mg/kg），持续给药3 d，空白对照组和单纯照射组给予相同体积的生理盐水。照射3 d后取材，观察各组小鼠脾、肺、肾、睾丸、肝脏等脏器指数变化，检测各组小鼠血清和肝组织中还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和丙二酮（MDA）含量。结果与单纯照射组比较，照射给药组肝组织中GSH含量和血清中SOD值升高，血清中MDA水平降低，差异均有统计学意义（P < 0.05）。结论百里醌可降低辐射小鼠中氧化损伤，对小鼠辐射损伤有一定的防护作用。

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	侯沁莲董银萍董辉李德冠杜利清

关键词 ：百里醌, 辐射防护, 氧化损伤

Abstract：Objective To observe the protective effects of thymoquinone (TQ) against radiation injuries in mice. Methods C57BL/6J mice were randomly divided into three group (n=5): control group, irradiation group and irradiation + TQ group. The mice in the irradiation group and the irradiation + TQ group were accepted 7.5 Gy total body irradiation, and the mice in the control group was received sham irradiation. The irradiation + TQ group was administered orally the day before exposure (10 mg/kg), sustained administration for 3 days. The same volume normal saline was given to the mice in the control and irradiation group. Three days after irradiation, the samples were taken, and the indexes of spleen, lung, kidney, testes and liver of the mice were observed, and the contents of GSH, SOD and MDA in serum and liver tissues of each group were detected. Results Compared with the irradiation group, the GSH content in the liver tissues and SOD value in the serum in the irradiation + TQ group were increased, and the MDA level in the serum was decreased, with statistically significant difference (P < 0.05). Conclusion TQ can reduce radiation oxidation damage in the radiation mice, and it has certain protective effect on the radiation injury in mice.

Key words： Thymoquinone Radiation protective Oxidative damage

基金资助:国家自然科学基金青年基金项目（31300601）；
“协和青年基金”项目（33320140186、3332016099）；
中国医学科学院医学与健康科技创新工程资助项目（2017-I2M-3-019）。

通讯作者: 杜利清（1973.6-），女，副研究员；研究方向：放射医学专业。

作者简介: 侯沁莲（1990.6-），女，中国医学科学院放射医学研究所2015级在读硕士研究生；研究方向：放射医学。

引用本文:

侯沁莲董银萍董辉李德冠杜利清. 百里醌对小鼠辐射防护作用[J]. 中国医药导报, 2018, 15(3): 4-7.
HOU Qinlian DONG Yinping DONG Hui LI Deguan DU Liqing . Radiation- protective effect of thymoquinone in mice. 中国医药导报, 2018, 15(3): 4-7.

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