Abstract：Objective To explore the effective ingredients and intervention mechanism of Yinqi Ointment in the treatment of diabetic foot ulcer, and to provide new ideas and methods for the development of new drugs. Methods The key target network of Yinqi Ointment in the treatment of diabetic foot ulcer was constructed with chemical composition data of Yinqi Ointment, candidate disease targets for diabetic foot ulcer, protein-protein interaction between information between potential targets of Yinqi Ointment and diabetic foot ulcer disease targets which were obtained from integrative pharmacology-based research platform of traditional Chinese medicine. Then the key targets and pathways of Yinqi Ointment in the treatment of diabetic foot ulcer were found by using the results of gene ontology database and enrichment analysis. Results Four key drug targets of Yinqi Ointment were found and the most key target for Yinqi Ointment in the treatment of diabetic foot ulcer might be HSP90AA1. The synthesis, recycling, salvage and regulation of tetrahydrobiopterin (BH4), endothelial nitric oxide synthase activation, and vascular endothelial cell growth factor receptor 2 mediated vascular permeability were the most important pathways for the treatment of diabetic foot ulcer. Conclusion Although this study provides a basis for the further study of Yinqi Ointment and its Chinese herbal ingredients, it is only based on the prediction of the integrated pharmacology platform. The specific mechanism and the development of new drugs need to be supported by specific laboratory research.
陈润铭 杜玉青 李友山 董学宇 安琪 谢存香. 基于网络药理学角度探讨银芪软膏对糖尿病足溃疡的干预机制[J]. 中国医药导报, 2021, 18(7): 4-8,17.
CHEN Runming DU Yuqing LI Youshan DONG Xueyu AN Qi XIE Cunxiang. Discussing the intervention mechanism of Yinqi Ointment on diabetic foot ulcer from the perspective of network pharmacology. 中国医药导报, 2021, 18(7): 4-8,17.
[1] 中华医学会糖尿病学分会，中华医学会感染病学分会，中华医学会组织修复与再生分会.中国糖尿病足防治指南（2019版）（Ⅰ）[J].中华糖尿病杂志，2019，11（2）：92-107.
[2] 李友山，刘凤桐，杜玉清，等.银芪软膏外治糖尿病足溃疡对创面形态及生长因子的影响[J].世界中医药，2019， 14（5）：1217-1221.
[3] 桑滨生.《中医药发展战略规划纲要（2016-2030年）》解读[J].世界科学技术-中医药现代化，2016，18（7）：1088-1092.
[4] 杨坤.网络药理学:新药物研发的新思路[A].中国药学会医院药学专业委员会、《中国医院药学杂志》.2015年全国医院药学（药学监护）学术会议论文集[C].中国药学会医院药学专业委员会、《中国医院药学杂志》：中国医院药学杂志编辑部，2015，1.
[5] Luo TT，Lu Y，Yan SK，et al. Network Pharmacology in Research of Chinese Medicine Formula：Methodology，Application and Prospective [J]. Chin J Integr Med，2020，26（1）：72-80.
[6] Hao da C，Xiao PG. Network pharmacology：a Rosetta Stone for traditional Chinese medicine [J]. Drug Dev Res，2014， 75（5）：299-312.
[7] 詹桂桂，宋潇萌，朱旅云.糖尿病足感染的发病机制及病原菌特征研究进展[J].临床误诊误治，2018，31（12）：105-109.
[8] 李永伟，王志盛，刘心伟.金银花抗感染作用研究进展[J].中国现代医生，2019，57（12）：165-168.
[9] 郭成成，张晓圆，玉应香，等.金银花提取物对高脂饲料诱导的肥胖大鼠糖耐量及其葡萄糖峰值时间的影响[C].中国营养学会第十四届全国营养科学大会暨第十一届亚太临床营养大会论文集.南京：中国营养学会，2019，424-425.
[10] 马园园，王静，罗琼，等.黄芪总皂苷药理作用研究进展[J].辽宁中医药大学学报，2020，22（7）：153-157.
[11] 杜玉青，刘亚莉，李友山，等.三七、黄芪总皂苷联合骨髓干细胞移植对糖尿病溃疡miRNA-146a的影响[J].世界中医药，2020，15（19）：2882-2887.
[12] Liu XY，Xu L，Wang Y，et al. Protective effects of total flavonoids of Astragalus against adjuvant-induced arthritis in rats by regulating OPG/ＲANKL/NF-κB pathway [J]. Int Immunopharmacol，2017（44）：105-114.
[13] Zhou YJ，Chen JM，Sapkota K，et al. Pananx notoginseng saponins attenuate CCL2-induced cognitive defificits in rats via anti-inflflammation and anti-apoptosis effffects that involve suppressing over-activation of NMDA receptors [J]. Biomed Pharmacother，2020，127：110139.
[14] 高爱社，李春花，王榭延.三七总皂苷对人脐静脉内皮细胞损伤后活性的影响[J].中医学报，2017，32（10）：1959-1961.
[15] 赵亮，张丽，曾强，等.ABL1相关融合基因与急性淋巴细胞性白血病关系的研究进展[J].中国慢性病预防与控制，2016，24（5）：383-385.
[16] Crabtree MJ，Channon KM. Synthesis and recycling of tetrahydrobiopterin in endothelial function and vascular disease [J]. Nitric Oxide，2011，25（2）：81-88.
[17] 卢晓云.四氢生物蝶呤在糖尿病性受损内皮细胞糖代谢中的作用[J].中国医药指南，2015，13（7）：52-53.
[18] 马莉.VEGF及其受体的生物学特性及在肿瘤血管生成中的作用[J].中国优生与遗传杂志，2016，24（5）：146-148.
[19] Tian H，Huang JJ，Golzio C，et al. Endoglin interacts with VEGFR2 to promote angiogenesis [J]. FASEB J，2018，32（6）：2934-2949.
[20] Venugopal S，Kao C，Chandna R，et al. Angio-3，a 10-residue peptide derived from human plasminogen kringle 3，suppresses tumor growth in mice via impeding both angiogenesis and vascular permeability [J]. Angiogenesis，2018，21（3）：653-665.
[21] Fan G，Tu Y，Wu N，et al. The expression profiles and prognostic values of HSPs family members in Head and neck cancer [J]. Cancer Cell Int，2020，20：220.
[22] Song KH，Oh SJ，Kim S，et al. HSP90A inhibition promotes anti-tumor immunity by reversing multi-modal resistance and stem-like property of immune-refractory tumors [J]. Nat Commun，2020，11（1）：562.
[23] Valerio M，Awad AB. β-Sitosterol down-regulates some pro-inflammatory signal transduction pathways by increasing the activity of tyrosine phosphatase SHP-1 in J774A. 1murine macrophages [J]. Int Immunopharmacol，2011，11（8）：1012-1017.
[24] 张帆.植物甾醇的生物活性与构效关系初探[D].南昌：南昌大学，2018：11-26.
[25] 王旭红，刘改霞，李耀辉，等.基于网络药理学探寻β-谷甾醇作用机制研究[J/OL].世界中医药：1-8[2021-02-04].http：//kns.cnki.net/kcms/detail/11.5529.R.202010 26.1244.004.html.
[26] Liao PC，Lai MH，Hsu KP，et al. Identification of β-Sitosterol as in Vitro Anti-Inflammatory Constituent in Moringa oleifera [J]. J Agric Food Chem，2018，66（41）：10748-10759.
[27] Ponnulakshmi R，Shyamaladevi B，Vijayalakshmi P，et al. In silico and in vivo analysis to identify the antidiabetic activity of beta sitosterol in adipose tissue of high fat diet and sucrose induced type-2 diabetic experimental rats [J]. Toxicol Mech Methods，2019，29（4）：276-290.
2021, Vol. 18 
京公网安备 11010502046598号