Preparation and targeted evaluation of Gαi2 C-terminal peptide targeted-ultrasound contrast agent
JIANG Hua1 MU Yuming2 WANG Chunmei2 ZHOU Xianhui3 ZHANG Jian3
1.Department of Geriatrics, Affiliated Hospital of Chengdu University, Sichuan Province, Chengdu 610081,China;
2.Department of Echocardiograrhy, Ultrasonic Medical Center, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China;
3.Heart Center， the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China
Abstract：Objective To explore the feasibility and targeting of Gαi2 C-terminal peptide (Gαi2ctp) targeted-ultrasound contrast agent. Methods The targeted microbubbles were divided into three groups of 0.5, 1.5, 3 mg/L according to the quality of Gαi2ctp. Gαi2ctp-targeted microbubbles were prepared by electrostatic attraction way, and the optimal combined dose was explored by detecting the size, carrying rate of microbubbles. The best dose was choosed for animal experiments by making a canine atrial fibrillation model. Ultrasound-targeted microbubble destruction was used to destroy the microbubbles and immunofluorescence method was used to detect the targeting of the microbubbles. Results Gαi2ctp and SonoVue microbubbles could be combined effectively by electrostatic attraction way. The Gαi2ctp targeted microbubble carrying rate of 1.5 mg/L group was higher than that of 3 mg/L group at 0.5, 1 and 2 h after standing, and the differences were highly statistically significant (all P < 0.01). There was no significant difference in the Gαi2ctp targeted microbubble carrying rate between 1.5 mg/L group and 0.5 mg/L group after 0.5 mg/L at 0.5, 1 and 2 h (P > 0.05). There was no statistically significant difference in the carrying rate of 1.5 mg/L group before and after washing (P > 0.05). After washing, the Gαi2ctp targeted microbubble carrying rate of 3 mg/L group and 0.5 mg/L group were lower than those before washing, and the differences were highly statistically significant (all P < 0.01). Frozen sections of canine left atrium and pulmnoary vein were showed that bright orange-red fluorescence expression in the left atrium and pulmnoary vein of dogs in experimental group (carrying 1.5 mg/L Gαi2ctp targeting microbubbles group), but no orange-red fluorescence was in control group (SonoVue blank group). Conclusion In this study, Gαi2ctp-targeted ultrasound contrast agent is successfully prepared. It is uniform in size, stable in nature, and specifically binded to target tissues.
2021, Vol. 18 
京公网安备 11010502046598号