Effect of ERH gene on human bladder cancer cell cycle
ZHOU Rongsheng1,2 HAN Conghui1,2,3 CHEN Bo1 HAO Lin1,3 SHI Zhenduo1 JIANG Bo1 ZHANG Zhiguo1,3 PANG Kun1,2,3▲
1.Department of Urology, Xuzhou Central Hospital Xuzhou Central Hospital Affiliated to Nanjing University of Chinese Medicine Xuzhou Hospital Affiliated to School of Medicine, Southeast University Xuzhou Clinical College of Xuzhou Medical University, Jiangsu Province, Xuzhou 221009, China;
2.College of Life Sciences, Jiangsu Normal University, Jiangsu Province, Xuzhou 221000, China;
3.Department of Urology, the Third Affiliated Hospital of Soochow University, Jiangsu Province, Changzhou 213000, China
Abstract：Objective To investigate the molecular biological mechanism of enhancer of rudimentary homolog (ERH) gene affecting proliferation and apoptosis of human bladder urothelial carcinoma (BUC) cell line T24. Methods BUC T24 cells were divided into ERH knockdown group and ERH normal cell group. The expression of genes affected by ERH knockdown in the whole gene spectrum was detected by human transcriptome expression profile microarray. The possible molecular biological mechanism of ERH gene affecting the proliferation and apoptosis of human BUC T24 cells was determined by bioinformatics analysis technology, biological functional enrichment and protein interaction analysis techniques, and was verified by functional experiments. Results The results of human transcriptome expression profile microarray technology showed that ERH gene expression was significantly decreased in ERH knockdown group, and the FC value was -4.50, P ＜ 0.0001. The expression of 344 genes in the ERH knockout group was up-regulated and 254 genes were down-regulated (cutoff value |FC|>2) compared with the ERH normal group. The results of biological function enrichment analysis indicated that ERH gene was closely related to cytokines, tumor necrosis factor, P53 down-regulation and apoptosis. The results of protein interaction analysis showed that ERH gene might affect the proliferation and apoptosis of BUC T24 cells through cyclin. Functional experiments suggested that the ERH gene produces these effects through the cell cycle, which mainly affects the S phase of the cell cycle. Conclusion ERH gene affects the proliferation and apoptosis of human bladder cancer T24 cells by regulating cell cycle.
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2020, Vol. 17 
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