Relationship between differential gene expression in pure oxygen and air pulmonary ventilation relating to lung injury
CHEN Chao1 LI Yuntao2 ZHANG Hongbo3 SHEN Liang4 NIE Xiaohu2 WEI Pengxiang5
1.Department of Anesthesia, Huzhou Central Hospital Affiliated Central Hospital of Huzhou University, Zhejiang Province, Huzhou 313000, China;
2.Department of Neurosurgery, Huzhou Central Hospital Affiliated Central Hospital of Huzhou University, Zhejiang Province, Huzhou 313000, China;
3.Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangdong Province, Guangzhou 510000, China;
4.Department of Neurosurgery, the Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, Jiangsu Province, Changzhou 213000, China; 5.Department of Neurosurgery, Beihai People’s Hospital, Guangxi Zhuang Autonomous Region， Beihai 536000, China
Abstract：Objective To explore the relationship between differential gene expression in pure oxygen and air pulmonary ventilation relating to lung injury. Methods Five Rattus norvegicus rats were randomly assigned to the experiment group (pure oxygen ventilations, n = 3) and the control group (air ventilation, n = 2). The R software was used to analyze the differential gene expression of lung tissues between the two groups, and functional enrichment was used to analyze differential gene function, protein-protein interactions were constructed to visualize and visualize the protein network of differential genes. Results There were 140 differential genes expressed in the lung tissues of two groups. The differential gene function was mainly enriched in mitochondria, mitochondrial respiratory chain complex Ⅰ, activation of homologous dimer proteins, intercellular connection and exosomes, among which mitochondria respiratory chain complex Ⅰ was a low-expression gene, which include NDUFB3, NDUFA4, NDUFB1, NDUFB9, NDUFB10, NDUFC1 and NDUFC2, respectively. Conclusion Lung injury under high oxygen content ventilation mainly inhibits the synthesis of ATP in mitochondrial respiratory chain complex Ⅰ. NDUFA4, NDUFB1, NDUFB3, NDUFB9, NDUFB10, NDUFC1, and NDUFC2 genes are the major differential genes in oxidative stress injury and play an important role in lung injury.
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2020, Vol. 17 
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