Application value of digital oil drop PCR method and ARMS method in detecting BRAF gene mutation in colorectal cancer
JIANG Yanping1 XUN Yanping1 FU Jiayi1 ZHANG Shirong1 ZHAO Yanyan1 MA Shenglin2
1.Translational Medicine Research Center, Affiliated Hangzhou First People′s Hospital, Zhejiang University School of Medicine, Zhejiang Province, Hangzhou 310006, China;
2.Department of Oncology, Affiliated Hangzhou First People′s Hospital, Zhejiang University School of Medicine, Zhejiang Province, Hangzhou 310006, China
Abstract：Objective To explore the application value of digital oil drop polymerase chain reaction (ddPCR) and amplification hindered mutation system (ARMS) in the detection of serine/threonine protein kinase (BRAF) V600E mutation in colorectal cancer patients. Methods Surgical specimens of 162 patients with colorectal cancer treated in Affiliated Hangzhou First People′s Hospital, Zhejiang University School of Medicine from April 2018 to July 2019 were collected. ARMS method and ddPCR method were used to detect BRAF V600E mutation in colorectal cancer patients, and the positive rate and consistency of ARMS and ddPCR were compared. The relationship between BRAF V600E mutation and clinicopathological characteristics of patients with colorectal cancer were analyzed. Results The positive rates of BRAF V600E mutation detected by ARMS and ddPCR were 8.64% (14/162) and 9.26% (15/162). High-throughput sequencing was performed on one case of the ambiguity of the BRAF V600E mutation detected by the ARMS method, and the result of the ddPCR method was consistent with the high-throughput sequencing result. Corresponding to the ddPCR, the positive coincidence rate, negative coincidence rate and consistency of the ARMS method were 93.33%, 99.32% and 99.38%, respectively. The patients who were higher the age, the poorly differentiated histological type, in clinical stages Ⅲ-Ⅳ, the higher the distribution of BRAF V600E mutations, and the differences were statistically significant (P ＜ 0.05). Conclusion Both ddPCR and ARMS can be used to detect BRAF V600E mutations in colorectal cancer, and ddPCR has higher detection efficiency.
2020, Vol. 17 
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