Relationship between high risk human papillomavirus infection and vaginal microecology and cervical local cellular immunity
HUANG Minmin1 YIN Zhifa2 QIN Xinlei1
1.Department of Gynecology, the Second People′s Hospital of Lianyungang, Jiangsu Province, Lianyungang 222023, China;
2.Department of Stomatology, the Second People′s Hospital of Lianyungang, Jiangsu Province, Lianyungang 222023, China
Abstract：Objective To explore the relationship between high risk human papillomavirus (HR-HPV) infection and vaginal microecology and cervical local cellular immunity. Methods One hundred and sixty patients with human papillomavirus (HPV) infection treated in the Second People′s Hospital of Lianyungang (“our hospital” for short) from February 2017 to August 2019 were divided into non HR-HPV group (85 cases) and HR-HPV group (75 cases) according to whether the patients were accompanied with HR-HPV infection. At the same time, 80 healthy women who had physical examination in our hospital at the same time were selected as control group. The vaginal microecology was detected by enzyme chemical reaction method, HPV-DNA was detected by fluorescence probe hybridization capture technology, and the proportion of positive cells of leukocyte differentiation antigen 3+ (CD3+), leukocyte differentiation antigen 4+ (CD4+) and leukocyte differentiation antigen 8+ (CD8+) were detected by flow cytometry. The incidence of vaginal microecology, the distribution of vaginal flora and the distribution of T lymphocyte subsets among groups were compared. Results HR-HPV patients were mainly single type of HPV infection, and HPV16, 18 and HPV33 were the main types. The incidence of vaginal microecological disorder in HR-HPV group was higher than that in non HR-HPV group and control group, while that in non HR-HPV group was higher than that in control group (P < 0.05). The positive rates of Lactobacillus abnormality, bacterial vaginitis, Chlamydia, Ureaplasma urealyticum and trichomonas in HR-HPV group and non HR-HPV group were significantly higher than those in control group (P < 0.05). The positive rates of Lactobacillus and Chlamydia in HR-HPV group were significantly higher than those in non HR-HPV group (P < 0.05). CD3+, CD4+ and CD4+/CD8+ in HR-HPV group were lower than those in non HR-HPV group and control group, while CD3+, CD4+ and CD4+/CD8+ in non HR-HPV group were lower than those in control group (P < 0.05). CD8+ in HR-HPV group was higher than that in non HR-HPV group and control group, while CD8+ in non HR-HPV group was higher than that in control group (P < 0.05). Conclusion HR-HPV infection can cause serious imbalance of vaginal microecology, increase the distribution of vaginal flora and further reduce the number of T-lymphocyte subsets, resulting in the deterioration of patients′ condition.
2020, Vol. 17 
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