Mechanistic study of Scutellariae Barbatae Herba in the treatment of pancreatic cancer by using network pharmacology
XU Yachen1 XIE Yiqiang2 XIAO Man2 ZHANG Rui2 SUN Zaoxi1
1.Department of Hepatobiliary Surgery, the First Affiliated Hospital, Hainan Medical University, Hainan Province, Haikou 570102, China;
2.College of Traditional Chinese Medicine, Hainan Medical University, Hainan Province, Haikou 571199, China
Abstract：Objective To study the mechanism of action of Scutellariae Barbatae Herba in treating pancreatic cancer by using network pharmacology. Methods Based on TCMSP, NPASS, HIT, TCMID, TCM-ID, CNKI, WANFANG DATA, CQVIP, Science Citation Index (SCI), PubMed databases. The active constituents of Scutellariae Barbatae Herba were searched from June 1971 to October 2019. The prediction platform was used to acquire and screen target genes. Venn analysis was used to screen interaction genes between component target genes and pancreatic cancer specific genes, then determined the key targets of the drug through drug-target-disease cross-linking. Then fully utilized gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) signal pathway enrichment to analyze molecular mechanism of Scutellariae Barbatae Herba in treating the disease. Results A total of 24 kinds of drug-like compounds were obtained, 73 target genes were determined, five core components including baicalein, wogonin, baicalin, quercetin, stigmasterol, and ten key targets such as AKT1, HSP90A1, tumor necrosis factor, ESR1, epidermal growth factor receptor, post-transcriptional gene silencing 2, SRC, matrix metalloproteinase 9, matrix metalloproteinase 2 and AR were screened out. GO enriched in the regulation of apoptosis, inflammation and oxidative stress, and were involved in the specific binding of proteins, ATPases and DNA. KEGG enriched in cancer, pancreatic cancer, cancer proteoglycan, Ras, phosphatidylinositol-3-kinase-protein kinase B pathway. Conclusion Scutellariae Barbatae Herba may regulate multiple signaling pathways through multiple types of targets, affecting multiple function of cells, thus playing a role in the treatment of pancreatic cancer.
徐亚晨1 谢毅强2 肖曼2 张瑞2 孙早喜1. 基于网络药理学的半枝莲治疗胰腺癌的作用机制研究[J]. 中国医药导报, 2020, 17(29): 123-127,146.
XU Yachen1 XIE Yiqiang2 XIAO Man2 ZHANG Rui2 SUN Zaoxi1. Mechanistic study of Scutellariae Barbatae Herba in the treatment of pancreatic cancer by using network pharmacology. 中国医药导报, 2020, 17(29): 123-127,146.
[1] Liu Q，Liao Q，Zhao Y. Chemotherapy and tumor microenvironment of pancreatic cancer [J]. Cancer Cell Int，2017， 17（68）：64-68.
[2] Kamisawa T，Wood LD，Itoi T，et al. Pancreatic cancer [J]. Lancet，2016，388（10039）：73-85.
[3] Dai ZJ，Lu WF，Jie G，et al. Anti-angiogenic effect of the total flavonoids in Barbated skullcup herb [J]. BMC Complement Altern Med，2013，13（1）：150-159.
[4] Wang TS. A review of phytochemistry and antitumor activity of a valuable medicinal species：Scutellaria barbata [J]. J Med Plants Res，2012，6（26）：4259-4275.
[5] 韩连强，谭杨，杨奇，等.胰腺癌治疗进展[J].中华消化病与影像杂志：电子版，2019，9（2）：49-54.
[6] Su MQ，Zhou YR，Rao X，et al. Baicalein induces the apoptosis of HCT116 human colon cancer cells via the upregulation of DEPP/Gadd45a and activation of MAPKs [J]. Int J Oncol，2018，53（2）：750-760.
[7] Yu X，Cao Y，Tang L，et al. Baicalein inhibits breast cancer growth via activating a novel isoform of the long noncoding RNA PAX8-AS1-N [J]. J Cell Biochem，2018，119（8）：6842-6856.
[8] Su G，Chen H，Sun X. Baicalein suppresses non-small cell lung cancer cell proliferation，invasion and Notch signaling pathway [J]. Cancer Bio，2018，22（1）：13-18.
[9] Ikemoto S，Sugimura K，Yoshida N，et al. Antitumoreffects of Scutellariae radix and its components baicalein， baicalin， and wogonin on bladder cancer cell lines [J]. Urology，2000，55（6）：951-955.
[10] Lee WR，Shen SC，Lin HY，et al. Wogonin and fisetin induce apoptosis in human promyeloleukemic cells，accompanied by a decrease of reactive oxygen species，and activation of caspase 3 and Ca（2+）-dependent endonuclease [J]. Bio Pharm，2002，63（2）：225-236.
[11] Chang WH，Chen CH，Lu FJ. Different effects of baicalein，baicalin and wogonin on mitochondrial function，glutathione content and cell cycle progression in human hepatoma cell lines [J]. Planta Med，2002，68（2）：128-132.
[12] Zhao Q，Wang J，Zou MJ，et al. Wogonin potentiates the antitumor effects of low dose 5-fluorouracil against gastric cancer through induction of apoptosis by down-regulation of NF-kappaB and regulation of its metabolism [J]. Toxicol Lett， 2010，197（3）：201-210.
[13] Kim H，Kim YS，Kim SY，et al. The plant flavonoid wogonin suppresses death of activated C6 rat glial cells by inhibiting nitric oxide production [J]. Neurosci Lett，2001，309（1）：67-71.
[14] 陈双秀，刘刚，李先霞.汉黄芩素对胰腺癌作用的体外、体内实验研究[J].中国普通外科杂志，2013，22（9）：1152-1157.
[15] Mi X，Zhu R. Simultaneous determination of 7 active ingredients in Scutellaria barabata D. Don by capillary micellar electrokinetic chromatography [J]. OALib J，2010， 28（2）：209-214.
[16] 刘俊，江弢，黄陈，等.Akt在胰腺癌中的研究进展[J].中华临床医师杂志：电子版，2010，4（9）：1639-1642.
[17] 赵爽，陈双双，于正洪.ESR1基因改变在乳腺癌内分泌治疗耐药中的研究进展[J].临床肿瘤学杂志，2016，21（10）：947-951.
[18] 钟志惟，殷香保.胰腺癌的分子靶向治疗研究进展[J].中国普通外科杂志，2016，25（9）：1351-1356.
[19] Biscardi JS，Maa MC，Tice DA，et al. Src-mediated phos-phorylation of the epidermal growth factor receptor on Tyr845 and Tyr1101 is associated with modulation of receptor function [J]. J Biol Chem，1999，274（1）：8335-8343.
[20] 韩贺.PGE2/AKT/p53轴调控化疗后胰腺癌细胞干性转化、增殖及其机制研究[D].江苏：江苏大学，2019.
[21] 綦俊.MMP2、MMP9、VEGF在胰腺癌中的表达及临床意义[D].长春：吉林大学，2012.
[22] 李玉军，姜天福，许加刚.胰腺癌雄激素受体的临床病理研究[J].中国肿瘤临床，1997，5（4）：306-307.
2020, Vol. 17 
京公网安备 11010502046598号