蛇葡萄素对实验性结肠炎大鼠Treg/Th17细胞分化的影响

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摘要目的探讨蛇葡萄素对实验性结肠炎大鼠Treg/Th17细胞分化的影响。方法 48只雄性SD大鼠采用随机数字表法分为对照组，模型组、高、中、低剂量组和柳氮磺吡啶组，每组8只。除对照组外，其余5组大鼠均使用三硝基苯磺酸建立实验性结肠炎模型。造模3 d后，药物治疗7 d，高、中、低剂量组大鼠分别灌胃给予500、250、125 mg/（kg·d）蛇葡萄素，柳氮磺吡啶组灌胃柳氮磺吡啶100 mg/（kg·d），对照组灌胃定量生理盐水。比较6组转化生长因子-β1（TGF-β1）、白细胞介素-10（IL-10）、IL-17、叉头转录因子3（Foxp3）、信号转导与转录活化因子5（STAT5）、维甲酸相关孤核受体γt（RORγt）、STAT3、IL-35表达水平。结果模型组病理损伤评分明显高于对照组（P ＜ 0.05）；高、中剂量组和柳氮磺吡啶组病理损伤评分低于模型组（均P ＜ 0.05）。模型组TGF-β1、IL-10、IL-35、Foxp3、STAT5表达水平低于对照组，IL-17、RORγt、STAT3表达水平高于对照组（均P ＜ 0.05）。高、中、低剂量组和柳氮磺吡啶组TGF-β1、IL-10、IL-35、Foxp3、STAT5表达水平高于模型组，IL-17、RORγt、STAT3表达水平均低于模型组（均P ＜ 0.05）。结论蛇葡萄素可以调节实验性结肠炎大鼠Treg/Th17细胞分化，增强细胞功能。

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	王超1 王晓娟1 朱美荣1 万玉刚2

关键词 ：蛇葡萄素, 实验性结肠炎, 白细胞介素, 转录因子, 调节性T细胞, 辅助性T-17细胞

Abstract：Objective To investigate the effect of Ampelopsin on the differentiation of Treg/Th17 cells in experimental colitis rats. Methods Forty-eight male SD rats were divided into control group, model group, high dose group, medium dose group, low dose group and Sulfasalazine group by random number table method, with eight rats in each groups. Except for control group, the other five groups of rats were used Trinitrobenzene Sulfonic Scid to establish experimental colitis model. After three days of modeling, drug treatment was given for seven days. Rats in high, medium, and low dose groups were given 500, 250 and 125 mg/(kg·d) of Ampelopsin respectively, and Sulfasalazine group was given 100 mg/(kg·d) of Sulfasalazine, and control group was given quantitative normal saline. The levels of transformed growth factor-β1 (TGF-β1), interleukin-10 (IL-10), IL-17, forkhead box P3 (Foxp3), signal transducers and activators of transcription 5 (STAT5), retinoic related orphan receptor γt (RORγt), STAT3 and IL-35 in six groups were compared. Results The pathological injury score of model group was higher than that of control group (P < 0.05); and the pathological injury scores of high, medium dose groups and Sulfasalazine group were lower than those of model group (all P < 0.05). The expression levels of TGF-β1, IL-10, IL-35, Foxp3 and STAT5 of model group were lower than those of control group, while the expression levels of IL-17, RORγt and STAT3 were higher than those of control group (all P < 0.05). The expression levels of TGF-β1, IL-10, IL-35, Foxp3 and STAT5 of high, medium, low dose groups and Sulfasalazine group were higher than those of model group, while the expression levels of IL-17, RORγt and STAT3 were lower than those of model group (all P < 0.05). Conclusion Ampelopsin can regulate Treg/Th17 cell differentiation and enhance cell function in experimental colitis rats.

Key words： Ampelopsin Experimental colitis Interleukin Transcription factor Regulatory T cells Helper T-17 cells

基金资助:山东省卫计委重点科技计划项目（GH20166867 587）。

引用本文:

王超1 王晓娟1 朱美荣1 万玉刚2. 蛇葡萄素对实验性结肠炎大鼠Treg/Th17细胞分化的影响[J]. 中国医药导报, 2020, 17(25): 19-23.
WANG Chao1 WANG Xiaojuan1 ZHU Meirong2 WAN Yugang3. Effect of Ampelopsin on the differentiation of Treg/Th17 cells in rats with experimental colitis. 中国医药导报, 2020, 17(25): 19-23.

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