Effect of Zhushui Yin on rats with malignant pleural effusion and its effect on expression of TGF-β1 and AQP-1 in rat pleural tissues
LIU Xiaofang1 WANG Xiaoting2 ZHAO Nan1▲
1.College of Acupuncture and Massage, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
2.Department of Oncology, Harbin Chinese Medicine Hospital, Heilongjiang Province, Harbin 150900, China
Abstract：Objective To investigate the therapeutic effect of Zhushui Yin on malignant pleural effusion in rats and its effect on expression of transforming growth factor-β1 (TGF-β1) and aquaporin-1 (AQP-1) in rat pleural tissues. Methods The rat model of malignant pleural effusion was established, and the successfully modeled rats were divided into five groups according to the random number table method, namely, the model group (MC), the positive control group (PC), the high-dose group (HD), the middle-dose group (MD), and the low-dose group (LD), with ten rats in each group. Another ten normal rats were recorded as normal group (NC). On the first day after modeling, PC was intraperitoneally injected with 2 mg/kg Cisplatin Injection once every two days. HD, MD and LD were respectively given 66.0, 33.0 g/kg and 16.5 g/kg of Zhushui Yin Liquid once a day. NC and MC were given the same volume of normal saline, once a day, for seven days in total. The amount of pleural effusion and the content of cancer cells in each group were compared. Pleural lesions were observed by hematoxylin-Eosin staining. The TGF-β1 and AQP-1 expression were compared in each group. Results The amount of pleural effusion and the number of cancer cells of MC, PC, HD, MD and LD on the fourth day and the eighth day were all higher than those on the first day, and the eighth day was higher than the fourth day. On the first day, the pleural effusion amount of MC rats was higher than NC, on the fourth day and the eighth day, the pleural effusion amount and cancer cell number of MC rats were higher than those of NC, while PC, HD, MD and LD were lower than MC, HD was lower than PC, LD was higher than PC, and the differences were statistically significant (all P ＜ 0.05). The pleural structure of NC rats was normal. The pleural thickening and infiltration of cancer cells and lymphocytes were serious in MC rats. The pleural thickness in HD, MD, LD was reduced and the infiltration of cancer cells was less. Compared with NC, the expressions of TGF-β1 and AQP-1 in MC were increased (P ＜ 0.05). Compared with MC, the expression levels of TGF-β1 and AQP-1 in PC, HD, MD and LD were all decreased (P ＜ 0.05). Compared with PC, the expression levels of TGF-β1 and AQP-1 in HD were decreased, while the expression levels of TGF-β1 and AQP-1 in LD were increased, with statistically significant differences (all P < 0.05). Conclusion Zhuishui Yin has a good effect on malignant pleural effusion in rats, which may be related to down-regulation of TGF-β1 and AQP-1 expression.
刘晓芳1 王晓婷2 赵楠1▲. 逐水饮治疗恶性胸腔积液大鼠的效果及对其胸膜组织TGF-β1、AQP-1表达的影响[J]. 中国医药导报, 2020, 17(21): 17-21.
LIU Xiaofang1 WANG Xiaoting2 ZHAO Nan1▲. Effect of Zhushui Yin on rats with malignant pleural effusion and its effect on expression of TGF-β1 and AQP-1 in rat pleural tissues. 中国医药导报, 2020, 17(21): 17-21.
[1] Meriggi F. Malignant Pleural Effusion：Still a Long Way to Go [J]. Rev Recent Clin Trials，2019，14（1）：24-30.
[2] Han YQ，Zhang L，Yan L，et al. Diagnostic accuracy of cancer ratio for malignant pleural effusion：a systematic review and meta-analysis [J]. Ann Transl Med，2019，7（20）：554-555.
[3] Terra RM，Costa PB，Dela Vega AJM，et al. Analysis of Quality of Life after Pleurodesis in Patients with Malignant Pleural Effusion [J]. Respiration，2019，98（6）：467-472.
[4] Thomas R，Roy B，Maldonado F，et al. Management of Malignant Pleural Effusions-What Is New [J]. Semin Respir Crit Care Med，2019，40（3）：323-339.
[5] 金志超，施展，花宝金.恶性胸腔积液的机制研究进展及中医药干预靶点的思考[J].世界中西医结合杂志，2017， 12（1）：11-17.
[6] 周立云，余小红，谢兆兰.扶正逐水饮与香菇多糖联合吡柔比星对恶性胸水患者免疫功能的影响[J].辽宁中医杂志，2012，39（11）：2209-2210.
[7] 杨子庆，张琦，徐路，等.温阳消饮法对胸腔积液大鼠小肠AQP4及cAMP-PKA信号通路表达的影响[J].中华中医药杂志，2016，31（7）：2765-2767.
[8] 李慧，严时，刘岩，等.晚期NSCLC患者恶性胸腔积液中上清和细胞沉淀EGFR基因突变状态的比较[J].国际肿瘤学杂志，2018，45（1）：10-15.
[9] Ferreiro L，Toubes ME，San José ME，et al. Advances in pleural effusion diagnostics [J]. Expert Rev Respir Med，2020，14（1）：51-66.
[10] 李枋霏，王维，张仲妍，等.葶苈大枣泻肺汤辅助化疗治疗肺癌恶性胸腔积液临床研究[J].国际中医中药杂志，2018，40（3）：214-217.
[11] Congcong Q，Hengting Z，Shuhui L，et al. Evaluation of Efficacy and Safety for Lentinan in the Control of the Malignant Pleural Effusions via Intrapleural Injection [J]. Am J Med Sci，2019，358（6）：400-411.
[12] 毕兰青，朱凡，张勤英，等.紫杉醇腹腔灌注和静脉滴注双途径给药模式二线治疗胃癌伴恶性腹腔积液的效果[J].中国医药，2018，13（1）：92-95.
[13] 李俊娇，葛信国.葛信国教授运用扶正逐饮法治疗肺癌恶性胸腔积液经验[J].中国中医急症，2018，27（1）：159-161.
[14] 李政，王巍，李康.葶苈甘遂逐水饮联合胸腔内灌注化疗治疗晚期非小细胞肺癌伴恶性胸水临床疗效分析[J].辽宁中医药大学学报，2016，18（1）：198-200.
[15] Dai B，Xiao Z，Mao B，et al. lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF-β pathway [J]. Oncol Lett，2019，18（6）：5923-5929.
[16] 吕大伦，徐姝娟，陈雷，等.人促红细胞生成素对大鼠急性创面转化生长因子β1/Smad3信号转导通路的影响[J].中华烧伤杂志，2018，34（10）：719-726.
[17] Wu DW，Chang WA，Liu KT，et al. Vascular endothelial growth factor and protein level in pleural effusion for differentiating malignant from benign pleural effusion [J]. Oncol Lett，2017，14（3）：3657-3662.
[18] Hojski A，Leitgeb M，Crnjac A. Release of growth factors after mechanical and chemical pleurodesis for treatment of malignant pleural effusion： a randomized control study [J]. Radiol Oncol，2015，49（4）：386-394.
[19] Liu M，Liu Q，Pei Y，et al. Aqp-1 Gene Knockout Attenuates Hypoxic Pulmonary Hypertension of Mice [J]. Arterioscler Thromb Vasc Biol，2019，39（1）：48-62.
[20] 杨格强，祝艳妮.沉默AQP-1对PDGF诱导的视网膜色素上皮细胞增殖迁移的影响研究[J].临床和实验医学杂志，2018，17（20）：2169-2173.
[21] Zhang JX，Xie CM，Zhu ZW，et al. Potential role of AQP1 and VEGF in the development of malignant pleural effusion in mice [J]. Med Oncol，2012，29（2）：656-662.
2020, Vol. 17 
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