Application value and clinical significance of elastica staining in visceral peritoneal elastic lamina invasion and vascular invasion of colorectal cancer
1.Department of Pathology, Qingdao University Medical College, Shandong Province, Qingdao 266071, China;
2.Department of Pathology, Qingdao Municipal Hospital, Shandong Province, Qingdao 266071, China
Abstract：Objective To explore the application value and clinical significance of elastica staining in identifying visceral peritoneal elastic lamina invasion and vascular invasion of colorectal cancer. Methods From January 2013 to December 2016, 75 colorectal cancer histological specimens were selected from Qingdao Municipal Hospital, the peritoneal invasion of the visceral layer, the peritoneal invasion of the visceral layer and the vascular invasion in the specimens were observed by conventional HE staining and lichen red elastic fiber staining. The relationship among lymph node metastasis, vascular invasion, nerve invasion and tumor budding were observed. Correlations between visceral peritoneal elastic lamina invasion and lymph metastasis, vascular invasion, perineural invasion, tumor budding were also investigated. Results Elastic fiber staining results showed the positive rate of visceral peritoneal elastic lamina invasion was higher than that of HE staining, and the difference was statistically significant (P ＜ 0.05); the incidence of lymph metastasis, vascular invasion and tumor budding in visceral peritoneal elastic lamina invasion positive cases were higher than those in peritoneal elastic lamina invasion negative cases, the differences were statistically significant (P ＜ 0.05); the incidence of nerve invasion in patients with positive visceral peritoneal elastic layer invasion was higher than that in patients without visceral peritoneal elastic layer invasion, and the difference was statistically significant (P ＜ 0.05); elastic fiber staining revealed that the detection rate of vascular invasion was higher than HE staining, and the difference was statistically significant (P ＜ 0.05), and with 100.00% specificity and the 65.8% sensitivity. Conclusion Elastic fiber staining can significantly improve the detection rate of visceral peritoneal elastic layer invasion and vascular invasion. Patients with positive visceral peritoneal elastic layer invasion suggest poor tumor prognosis, and visceral peritoneal elastic layer invasion may be one of the clinicopathological indicators for colorectal cancer staging.
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2020, Vol. 17 
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