Abstract：Bacterial infection has become a clinical situation that can't completely avoid, including pathogens and conditioned pathogens. Bacterial biofilm becomes an effective way to protect bacteria, increases the bacteria against the body immune defense and the resistance to antibiotics. Staphylococcus biofilm formation affected by multiple factors and multi-gene regulation, including the external environmental factors, such as organism immune response and external organic matter, the internal genes regulation are mainly sarA, agr, aap, ica. This paper summarizes the formation of Staphylococcal biofilm, the external environmental factors and internal gene regulation factors that affecting biofilm formation, which can be used as a reference for the study and treatment of Staphylococcal biofilm targeted drugs.
[1] Romling U，Balsalobre C. Biofilm infections，their resilience to therapy and innovative treatment strategies [J]. J Intern Med，2012，272（6）：541-561.
[2] Song F，Koo H，Ren D. Effects of Material Properties on Bacterial Adhesion and Biofilm Formation [J]. J Dent Res，2015，94（8）：1027-1034.
[3] Cos P，Tote K，Horemans T，et al. Biofilms：an extra hurdle for effective antimicrobial therapy [J]. Curr Pharm Des，2010， 16（20）：2279-2295.
[4] Srivastava S，Bhargava A. Biofilms and human health [J]. Biotechnol Lett，2016，38（1）：1-22.
[5] Percival SL. Importance of biofilm formation in surgical infection [J]. Br J Surg，2017，104（2）：e85-e94.
[6] Rabin N，Zheng Y，Opoku-Temeng C，et al. Biofilm formation mechanisms and targets for developing antibiofilm agents [J]. Future Med Chem，2015，7（4）：493-512.
[7] Rowson C，Townsend R. Biofilms：prevention and treatment [J]. Br J Hosp Med，2016，77（12）：699-703.
[8] Berlanga M，Guerrero R. Living together in biofilms：the microbial cell factory and its biotechnological implications [J]. Microb Cell Fact，2016，15（1）：165.
[9] Feraco D，Blaha M，Khan S，et al. Host environmental signals and effects on biofilm formation [J]. Microb Pathog，2016，99：253-263.
[10] Paharik AE，Horswill AR. The Staphylococcal Biofilm：Adhesins，Regulation，and Host Response [J]. Microbiol Spectr，2016，4（2）：10.
[11] Nguyen TH，Park MD，Otto M. Host Response to Staphylococcus epidermidis Colonization and Infections[J].Front Cell Infect Microbiol，2017，7：90.
[12] Kristian SA，Birkenstock TA，Sauder U，et al. Biofilm formation induces C3a release and protects Staphylococcus epidermidis from IgG and complement deposition and from neutrophil-dependent killing [J]. J Infect Dis，2008， 197（7）：1028-1035.
[13] Dapunt U，Giese T，Stegmaier S，et al. The osteoblast as an inflammatory cell：production of cytokines in response to bacteria and components of bacterial biofilms [J]. BMC Musculoskelet Disord，2016，17（1）：243.
[14] Marriott I，Gray DL，Tranguch SL，et al. Osteoblasts express the inflammatory cytokine interleukin-6 in a murine model of Staphylococcus aureus osteomyelitis and infected human bone tissue [J]. Am J Pathol，2004，164（4）：1399-1406.
[15] Fitzgerald T，Yotis WW. Hormonal intervention in the uptake of amino acids by staphylococci [J]. J Med Microbiol，1971，4（1）：89-96.
[16] Pelzer ES，Allan JA，Theodoropoulos C，et al. Hormone-dependent bacterial growth persistence and biofilm formation—a pilot study investigating human follicular fluid collected during IVF cycles [J]. PLoS One，2012，7（12）：e49965.
[17] 王曦，黄云超，周永春，等.雌二醇对乳腺癌假体植入术后表皮葡萄球菌生物膜形成的影响研究[J].中国修复重建外科杂志，2016，30（7）：876-884.
[18] Knobloch JK，Bartscht K，Sabottke A，et al. Biofilm formation by Staphylococcus epidermidis depends on functional RsbU，an activator of the sigB operon：differential activation mechanisms due to ethanol and salt stress [J]. J Bacteriol，2001，183（8）：2624-2633.
[19] 申友亮，朱同娥，张靖靖，等.构建膝关节假体感染兔模型：体内环境因素对表皮葡萄球菌及生物膜的影响[J].中国组织工程研究，2015，19（39）：6240-6245.
[20] Artini M，Cicatiello P，Ricciardelli A，et al. Hydrophobin coating prevents Staphylococcus epidermidis biofilm formation on different surfaces [J]. Biofouling，2017，33（7）：601-611.
[21] 邱良婷，叶联华，黄云超.生物材料植入感染与表皮葡萄球菌生物膜形成的相关基因调控[J].国际生物医学工程杂志，2016，39（1）：37-42.
[22] Cheung AL，Nishina K，Manna AC. SarA of Staphylococcus aureus binds to the sarA promoter to regulate gene expression [J]. J Bacteriol，2008，190（6）：2239-2243.
[23] Buttner H，Mack D，Rohde H. Structural basis of Staphylococcus epidermidis biofilm formation：mechanisms and molecular interactions [J]. Front Cell Infect Microbiol，2015， 5（14）：14.
[24] 成鹏，叶联华.agrC对表皮葡萄球菌生物膜形成作用机制的研究进展[J].国际生物医学工程杂志，2016，39（4）：233-338.
[25] Snowden JN，Beaver M，Beenken K，et al. Staphylococcus aureus sarA regulates inflammation and colonization during central nervous system biofilm formation [J]. PLoS One，2013，8（12）：e84089.
[26] Beenken KE，Blevins JS，Smeltzer MS. Mutation of sarA in Staphylococcus aureus limits biofilm formation [J]. Infect Immun，2003，71（7）：4206-4211.
[27] Tormo MA，Marti M，Valle J，et al. SarA is an essential positive regulator of Staphylococcus epidermidis biofilm development [J]. J Bacteriol，2005，187（7）：2348-2356.
[28] Thoendel M，Kavanaugh JS，Flack CE，et al. Peptide signaling in the staphylococci [J]. Chem Rev，2011，111（1）：117-151.
[29] Laverty G，Gorman SP，Gilmore BF. Biomolecular mechanisms of staphylococcal biofilm formation [J]. Future Microbiol，2013，8（4）：509-524.
[30] Morfeldt E，Taylor D，von Gabain A，et al. Activation of alpha-toxin translation in Staphylococcus aureus by the trans-encoded antisense RNA，RNAⅢ [J]. Embo J，1995， 14（18）：4569-4577.
[31] Mootz JM，Benson MA，Heim CE，et al. Rot is a key regulator of Staphylococcus aureus biofilm formation [J]. Mol Microbiol，2015，96（2）：388-404.
[32] Lister JL，Horswill AR. Staphylococcus aureus biofilms：recent developments in biofilm dispersal [J]. Front Cell Infect Microbiol，2014，4（4）：178.
[33] Li S，Huang H，Rao X，et al. Phenol-soluble modulins：novel virulence-associated peptides of staphylococci [J]. Future Microbiol，2014，9（2）：203-216.
[34] Le KY，Dastgheyb S，Ho TV，et al. Molecular determinants of staphylococcal biofilm dispersal and structuring [J]. Front Cell Infect Microbiol，2014，4：167.
[35] 胡健.表皮葡萄球菌聚集相关蛋白（Aap）作为抗细菌生物膜疫苗候选抗原的研究[D].上海：复旦大学，2011.
[36] Paharik AE，Kotasinska M，Both A，et al. The metalloprotease SepA governs processing of accumulation-associated protein and shapes intercellular adhesive surface properties in Staphylococcus epidermidis [J]. Mol Microbiol，2017，103（5）：860-874.
[37] Conlon BP，Geoghegan JA，Waters EM，et al. Role for the A domain of unprocessed accumulation-associated protein（Aap）in the attachment phase of the Staphylococcus epidermidis biofilm phenotype [J]. J Bacteriol，2014，196（24）：4268-4275.
[38] Hussain M，Herrmann M，von Eiff C，et al. A 140-kilodalton extracellular protein is essential for the accumulation of Staphylococcus epidermidis strains on surfaces [J]. Infect Immun，1997，65（2）：519-524.
[39] Sun D，Accavitti MA，Bryers JD. Inhibition of biofilm formation by monoclonal antibodies against Staphylococcus epidermidis RP62A accumulation-associated protein [J]. Clin Diagn Lab Immunol，2005，12（1）：93-100.
[40] Arciola CR，Gamberini S，Campoccia D，et al. A multiplex PCR method for the detection of all five individual genes of ica locus in Staphylococcus epidermidis. A survey on 400 clinical isolates from prosthesis-associated infections [J]. J Biomed Mater Res A，2005，75（2）：408-413.
[41] 叶联华，黄云超，杨达宽，等.表皮葡萄球菌ica操纵子与聚氯乙烯材料表面细菌生物膜形成的关系[J].中华医院感染学杂志，2010，20（24）：3841-3843.
[42] Arciola CR，Campoccia D，Ravaioli S，et al. Polysaccharide intercellular adhesin in biofilm：structural and regulatory aspects [J]. Front Cell Infect Microbiol，2015，5（7）：7.
[43] O′Gara JP. ica and beyond：biofilm mechanisms and regulation in Staphylococcus epidermidis and Staphylococcus aureus [J]. Fems Microbiol Lett，2007，270（2）：179-188.
[44] Cue D，Lei MG，Lee CY. Genetic regulation of the intercellular adhesion locus in staphylococci [J]. Front Cell Infect Microbiol，2012，2（1）：38.
[45] Yu L，Hisatsune J，Hayashi I，et al. A Novel Repressor of the ica Locus Discovered in Clinically Isolated Super-Biofilm-Elaborating Staphylococcus aureus [J]. MBio，2017， 8（1）：e02282-16.
2017, Vol. 14 
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