降钙素基因相关肽受体拮抗剂对大鼠急性心肌缺血后心律失常的影响

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摘要 [摘要] 目的观察降钙素基因相关肽（CGRP）受体拮抗剂CGRP8-37对大鼠急性心肌缺血后室性心律失常的影响。方法选取健康成年雄性SD大鼠24只，体重250～300 g，将其随机分为三组（n = 8）：对照组（Sham组）、急性冠脉结扎（CAO）组、CGRP8-37预先干预急性冠脉结扎组（CGRP8-37-CAO组）。按单发、二联、三联和三次以上室性心律失常的类型统计CAO前10 min至CAO后60 min心律失常的发生次数并进行统计分析。结果 CAO可诱发大鼠室性心律失常的发生，发生时间主要集中在缺血早期的0～12 min，并且在CAO后6 min左右达到最高峰。CAO前用CGRP8-37预处理可明显增加大鼠室性心律失常的发生次数，并且发生的时程从CAO组的0～12 min延长到0～20 min，心律失常出现的高峰改变为CAO后12 min。Sham组、CAO组和CGRP8-37-CAO三组动物CAO前10 min室性心律失常发生次数比较差异无统计学意义（P ＞ 0.05）。与Sham组比较，CAO组、CGRP8-37-CAO组室性心律失常发生次数明显增多，差异有高度统计学意义（P ＜ 0.01）。与CAO组比较，CGRP8-37-CAO组室性心律失常发生次数也明显增多，差异有高度统计学意义（P ＜ 0.01）。室性心律失常出现差异的时间主要集中在CAO后20、30、40 min。结论 CGRP8-37可明显增加大鼠急性心肌缺血后室性心律失常的发生次数。

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	王利利张瑞林郭政▲

关键词 ： [关键词] 降钙素基因相关肽受体拮抗剂, 急性心肌缺血, 室性心律失常

Abstract：[Abstract] Objective To observe the effect of calcitonin gene-related peptide (CGRP) receptor antagonist CGRP8-37 on ventricular arrhythmia after acute myocardial infarction in rats. Methods Twenty-four healthy adult male Sprague-Dawley rats weighing 250-300 g were selected and randomly assigned into three groups (n = 8): control group (sham group), coronary artery occlusion group (CAO group) and CGRP8-37 intervention for coronary artery occlusion in advance group (CGRP8-37-CAO group). The times of ventricular arrhythmia from 10 min before CAO to 60 min after CAO were calculated and analyzed statistically according to the types of ventricular arrhythmia including one ventricular premature beat, two consecutive ventricular premature beats, three consecutive ventricular premature beats, more than three consecutive ventricular premature beats. Results CAO could induce the occurrence of ventricular arrhythmias, the occurrence time was concentrated on 0-12 min of early ischemia and peaked around 6 min after CAO. Pretreatment of CGRP8-37 before CAO could significantly increase the frequency of arrhythmias, and the time course was significantly extended from 0-12 min in CAO group to 0-20 min in CGRP8-37-CAO group. Peaked time of ventricular premature beats appeared in 12 min after CAO. The occurrence of ventricular arrhythmias in sham group, CAO group and CGRP8-37-CAO group had no significant differences 10 min before CAO (P ＞ 0.05). Compared with sham group, the occurrence of ventricular arrhythmias was increased in CAO group and CGRP8-37-CAO group, the differences were highly statistically significant (P ＜ 0.01). Compared with CAO group, the occurrence of ventricular arrhythmia in CGRP8-37-CAO group was increased, the difference was highly statistically significant (P ＜ 0.01). The time of differences appeared in ventricular arrhythmia was concentrated on 20, 30, 40 min after CAO. Conclusion CGRP8-37 can significantly increase the occurrence of ventricular arrhythmia after acute myocardial infarction in rats.

Key words： [Key words] Calcitonin gene-related peptide receptor antagonist Acute myocardial infarction Ventricular arrhythmias

基金资助:[基金项目] 山西省自然科学基金项目（2013011049-3）。

引用本文:

王利利张瑞林郭政▲. 降钙素基因相关肽受体拮抗剂对大鼠急性心肌缺血后心律失常的影响[J]. 中国医药导报, 2016, 13(3): 17-20.
WANG Lili ZHANG Ruilin GUO Zheng▲ . Effects of calcitonin gene-related peptide receptor antagonist on ventricular arrhythmia after acute myocardial infarction in rats. 中国医药导报, 2016, 13(3): 17-20.

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