The effect of copper metabolism domain containing 1 in U87 cells proliferation and apoptosis and mechanism study
RUAN Dong1 GUO Lirui2 WANG Fang2 YUAN Fan′en2 CHEN Qianxue2 LIU Baohui2
1.Department of Neurological Surgery, the First Affiliated Hospital of Hubei University of Science and Technology Xianning Central Hospital, Hubei Province, Xianning 437100, China;
2.Department of Neurological Surgery, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, China
Abstract：[Abstract] Objective To study the biological function of copper metabolism domain containing 1(COMMD1) in glioma cells U87 and its mechanism. Methods siRNA transfection was applied to knock down COMMD1 expression in U87 cells. CCK-8 and flow cytometry analysis were employed to examine glioma cells proliferation and apoptosis after COMMD1 was knocked down. Western blot was used to detect the expression of p65, cleaveage-caspase3 and BAD levels. Results Data showed that when COMMD1 was knocked down by siCOMMD1, the CCK8 results of siCOMMD1 were (0.402±0.000), (0.510±0.001), (1.021±0.002), (1.612±0.002), while the CCK8 results of siNC were (0.401±0.001), (0.452±0.002), (0.621±0.002), (0.823±0.003) at 0, 1, 2, 3 d; there were significantly differences in cell viabilities between the two group at 2 d and 3 d (P ＜ 0.05). Flow cytometry analysis results showed that the apoptosis rate of cells in siCOMMD1 group and siNC group was (2.214±0.325)% and (7.121±0.520)%, there was significantly difference between the two group (P ＜ 0.05). Western blot results showed that the expression of p65 and BAD increased 40.132% and 90.157%, cleaveage-caspase3 decreased 56.169% when COMMD1 was knocked down. Conclusion COMMD1 can regulate U87 cells apoptosis and proliferation.
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2017, Vol. 14 
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