Abstract：Objective To analysis inhibition of influenza A virus by influenza Shuangjiefang Decoction in vitro experimental. Methods Twenty-four New Zealand white rabbits were random divided into three groups, eight per group. Influenza Shuangjiefang Decoction, Ribavirin, and normal saline were given by gavage and drug-containing serum was prepared to analyze the effect of drugs on inhibiting influenza A virus. Results The influenza A virus rat lung adaptation strain A/PR8/34 (H1N1) TCID50 was 10-4.575/100 μL. The maximum non-toxic concentration of influenza Shuangjiefang Decoction and Ribavirin-containing serum were 40% and 50%, respectively. The experiments prevent infection, direct inactivation and replication proliferation inhibition showed that the OD value of the virus infection group was lower than that of the cell group (P ＜ 0.05); the OD value of the ribavirin group and the influenza Shuangjiefang Decoction group were higher than that of the virus infection group (P ＜ 0.05). There was no statistically significant difference in virus inhibition rate between the ribavirin group and the influenza Shuangjiefang Decoction group (P ＞ 0.05). Conclusion Influenza Shuangjiefang Decoction has the functions of in vitro prevention, direct killing and inhibition of influenza A virus after infection.
周雅萍 李国春 关晓伟. 流感双解方抑制甲型H1N1流感病毒的体外实验研究[J]. 中国医药导报, 2020, 17(12): 28-31.
ZHOU Yaping LI Guochun GUAN Xiaowei. Study on inhibition of influenza A virus by influenza Shuangjie Decoction in vitro experimental. 中国医药导报, 2020, 17(12): 28-31.
2020, Vol. 17 
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