The key genes in esophageal squamous cell carcinoma screened by bioinformatics
ZHAO Fei1 YUAN Zhiqing2
1.The Third Clinical College, Xinxiang Medical University, He′nan Province, Xinxiang 453003, China;
2.College of Basic Medicine, Xinxiang Medical University, He′nan Province, Xinxiang 453003, China
Abstract：Objective To provide new ideas for the study of tumor pathogenesis by screening the key genes of esophageal squamous cell carcinoma (ESCC). Methods The gene expression chips of ESCC were retrieved in GEO database. The differential expression genes were analyzed for obtaining common differential genes. GO and KEGG pathway enrichment analysis were performed in DAVID online database. The 10 key genes with the highest link were obtained by String database and Cytoscape software, and verified in the TCGA database. Results A total of 204 differentially expressed genes were screened. GO analysis showed that the biological process was enriched in 163 items, such as cell division, organelle fission, cell cycle, etc. Cytological components were enriched in 48 entries including extracellular, cytoplasmic and organelle lumen. Molecular function enrichment in 46 entries, such as regulating peptidase activity, binding to extracellular matrix, etc. KEGG pathways were enriched in 12 entries, including local adhesion, p53 signaling pathways, mismatch repair, etc. The 10 highest-linked Hub genes were verified to be highly expressed in ESCC by TCGA databases (P ＜ 0.01). Conclusion CDK1, CCNA2, RFC4, CCNB1, TOP2A, AURKA, CDC6, BUB1, BUB1B and PLK1 are the key genes of ESSC, and may be biomarkers and therapeutic targets in ESCC.
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