Repair effect of placental mesenchymal stem cells on endometrial injury in rats
WANG Xiangxiang1,2 WANG Yan1,2 YANG Shaoshao1,2 ZHANG Junjun2
1.College of Clinical Medical, He′nan University of Science and Technology, He′nan Province, Luoyang 471003, China;
2.Department of Obstetrics and Gynecology, the First Affiliated Hospital of He′nan University of Science and Technology, He′nan Province, Luoyang 471003, China
Abstract：Objective To study the repair effect of placental mesenchymal stem cells on endometrial injury in rats. Methods From May to November 2019, a total of 60 female SD rats (Qinglongshan Animal Breeding Farm, Jiangning District, Nanjing) were constructed endometrial injury models by chemical damage method, and they were divided into control group, gel group and model group by simple random sampling, with 20 rats in each group. After three estrous cycles, 10 rats from each group were randomly sampled for HE staining, and the number of endometrial glands and endometrial thickness were measured, while the percentage of endometrial fibrosis were measured by Masson staining. The other 30 rats were caged with male rats. On the 10th day of conception, the number of uterine embryos in the rats was observed by laparotomy. Results Compared with the control group, the number of endometrial glands decreased, the thickness of the endometrium became thinner, the percentage of endometrial fibrosis increased, while the number of uterine embryos in pregnant rats decreased in the model group, and the differences were statistically significant (P ＜ 0.05). Compared with the model group, the number of endometrial glands increased, the thickness of the endometrium increased, the percentage of endometrial fibrosis area decreased, while the number of uterine embryos in pregnant rats increased in the gel group, and the differences were statistically significant (P ＜ 0.05). Compared with the control group, the number of endometrial glands and endometrial thickness had no significant changes in the gel group, and the differences were not statistically significant (P ＞ 0.05); while the percentage of endometrial fibrosis increased, the number of uterine embryos in pregnant rats decreased, and the differences were statistically significant (P ＜ 0.05). Conclusion Placental mesenchymal stem cells can repair damaged endometrium, increase the number of endometrial glands and endometrial thickness, reduce the percentage of endometrial fibrosis area, and improve embryo implantation rate.
[1] Singh N，Mohanty S，Seth T，et al. Autologous stem cell transplantation in refractory Asherman′s syndrome：A novel cell based therapy [J]. J Hum Reprod Sci，2014，7（2）：93-98.
[2] 张婷，王颖，王莉.大鼠阴道涂片两种染色方法比较[J].中国比较医学杂志，2018，28（12）：101-104.
[3] 许春燕，宋阳，李坤寅，等.薄型子宫内膜模型的动物选择及其造模方法改进[J].中国实验动物学报，2016，24（2）：217-220.
[4] Haiyan Z，Yinshen J，Yibin P，et al. Human menstrual blood-derived stem cells promote the repair of impaired endometrial stromal cells by activating the p38 MAPK and AKT signaling pathways [J]. Reprod Biol，2018，18（3）：274-281.
[5] 牛婷，李爱斌，曹景云，等.胎盘间充质干细胞的应用研究[J].中国组织工程研究，2015，19（32）：5236-5242.
[6] Hur W，Lee HY，Min HS，et al. Regeneration of full-thickness skin defects by differentiated adipose-derived stem cells into fibroblast-like cells by fibroblast-conditioned medium [J]. Stem Cell Res Ther，2017，8（1）：92.
[7] Redondocastro E，Cunningham C，Miller J，et al. Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro [J]. Stem Cell Res Ther，2017，8（1）：79.
[8] Zhu J，Wang P，Yu Z，et al. Advanced glycosylation end product promotes forkhead box O1 and inhibits Wnt pathway to suppress capacities of epidermal stem cells [J]. Am J Transl Res，2016，8（12）：5569-5579.
[9] Vasandan AB，Jahnavi S，Shashank C，et al. Human Mesenchymal stem cells program macrophage plasticity by altering their metabolic status via a PGE2-dependent mechanism [J]. Sci Rep，2016，6（1）：38308.
[10] 王冰玉，姬霞.骨髓间充质干细胞移植联合益母草碱治疗宫腔粘连[J].中国组织工程研究，2016，20（32）：4771-4777.
[11] Wang J，Ju B，Pan C，et al. Application of Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Intrauterine Adhesions in Rats [J]. Cellular Physiol Biochem，2016，39（4）：1553-1560.
[12] Li B，Zhang Q，Sun J，et al. Human amniotic epithelial cells improve fertility in an intrauterine adhesion mouse model [J]. Stem Cell Res Ther，2019，10：257.
[13] 刘芳，朱志军，李福霞.骨髓间充质干细胞移植治疗宫腔粘连的体内实验研究[J].中国妇幼保健，2017，32（19）：4809-4813.
[14] 林鑫子，黄晨玲子，罗新，等.人早孕蜕膜间充质干细胞移植治疗大鼠宫腔粘连[J].中国病理生理杂志，2016， 32（6）：1099-1105.
[15] Gan L，Duan H，Xu Q，et al. Human amniotic mesenchymal stromal cell transplantation improves endometrial regeneration in rodent models of intrauterine adhesions [J]. Cytotherapy，2017，19（5）：603-616.
[16] Zhang L，Li Y，Guan CY，et al. Therapeutic effect of human umbilical cord-derived mesenchymal stem cells on injured rat endometrium during its chronic phase [J]. Stem Cell Res Ther，2018，9（1）：36.
[17] 艾瑛，廖诗平，王玉芳，等.脐带间充质干细胞移植预防宫腔粘连的研究[J].现代妇产科进展，2019，28（5）：24-27.
[18] 陶莹，吴敏敏，骆婕.人脐带间充质干细胞治疗大鼠子宫内膜损伤的研究[J].现代妇产科进展2019，28（7）：523-527.
[19] 牛婷，李爱斌，陈力.胎盘间充质干细胞修复受损子宫内膜[J].中国组织工程研究，2017，21（13）：2074-2080.
[20] 赵静.骨髓间充质干细胞移植治疗薄型子宫内膜的实验研究[D].长沙：中南大学，2013.
2020, Vol. 17 
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