Abstract：Objective To investigate the effect of Edaravone (Eda) combined with ischemic postconditioning (IP) on cerebral ischemia-reperfusion injury (I/R), and to evaluate whether Eda combined with IP have superimpose protective effects on I/R compared with IP alone. Methods Thirty-six SD rats were selected and divided into the control group (I/R group), IP group and IP+Eda group according to the random number table method, with 12 rats in each group. The rat I/R model was established in the I/R group by suture method. The rat I/R model was established by suture method in the IP group with IP intervention. The rat I/R model was established by suture method in the IP+Eda group with Eda and IP intervention. At 48 h after the operation, the rats were evaluated for neurological deficits and the volume of cerebral infarction was measured. By fluorescence quantitative PCR, Western blot and immunohistochemical method the expression of tissue angiotensin Ⅱ with type 1 and type 2 receptor (AT1, AT2) in the rat brain tissue was detected. The inhibition rate of superoxide dismutase (SOD) of rats in each group was detected. Results Compared with I/R group, IP+Eda group and IP group showed a decrease in neurological deficit score, a decrease in cerebral infarction volume, a decrease in mRNA and protein expression of AT1 and AT2, a decrease in the number of AT1 and AT2 positive cells, and an increase in SOD inhibition rate, with statistically significant differences (P ＜ 0.01 or P ＜ 0.05). There was no significant difference in the above indicators between the IP+Eda group and the IP group (P ＞ 0.05). Conclusion The IP+Eda group can protect rat I/R, but the combination of the two groups does not produce a superimposed protective effect.
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2020, Vol. 17 
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