Abstract：Objective To investigate the contents of the functional components in Fritillaria thunbergii from different geographical locations. Methods Kromasil ODS C18 column (200 mm × 4.6 mm, 5 μm) column was used as the column, acetonitrile-0.03% diethylamine was used as the mobile phase, the flow rate of 1.0 mL/min. The colomn temperature maintained at 40℃. Multivariate statistical analysis was carried out by SPSS 20.0. Results The total contents of verticine and verticinone were the highest in Longbu village, Yunhe county, Lishui city (LS-YH-LB). The content of verticine was higher than that of verticinone in 14 producing areas, and the verticinone was higher than that of verticine in 5 producing areas. In the cluster analysis, eight high-content producing areas, such as LS-YH-LB and Leqing city, Wenzhou city, were grouped into one category, while other producing areas were grouped into one category. Conclusion There are obvious geographical variations in the functional components of Fritillaria thunbergii, which indicats that there are some quality differences in different producing areas of Fritillaria thunbergii. This study provides reference for the standardized planting and further development and utilization of Fritillaria thunbergii.
张晓芹 吕虹艳 蓝艳 陈礼平. 基于HPLC-ELSD对浙贝母功效成分地理变异的研究[J]. 中国医药导报, 2020, 17(3): 110-114.
ZHANG Xiaoqin LYU Hongyan LAN Yan CHEN Liping. Quantification of chemical compositions in Fritillaria thunbergii from different geographical origin by HPLC-ELSD. 中国医药导报, 2020, 17(3): 110-114.
2020, Vol. 17 
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