Effect of matrine on RANKL-induced osteoclast differentiation and the expression of TRAF6, c-fos and NFATc1
LI Xin1 LI Fang2 WANG Yuan1 ZHANG Baoyu1 ZHANG Lijie1
1.Endocrine Metabolism and Immune Center, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, China;
2.Department of Rheumatology, the Second Hospital of Shanxi Medical University, Shanxi Province, Taiyuan 030001, China
Abstract：Objective To investigate the effect of matrine on RANKL-induced osteoclast differentiation and the possible molecular mechanism. Methods RAW264.7 cells were cultured. According to the different addition of drugs (none, osteoclast inducer RANKL and different doses of matrine), they were divided into blank group, induction group, low-dose matrine group (0.5 mg/mL), medium-dose matrine group (1 mg/mL) and high-dose matrine group (2 mg/mL). The expression of TRAF6, c-fos, NFATc1 mRNA and protein were detected by qPCR and Western blot at the 48th hour after administration. Multinucleated osteoclasts were observed by TRAP on the 7th day. The expression of Calcr and Ctsk mRNA was detected by qPCR on the 9th day. The bone resorption lacunae were observed by toluidine blue staining on the 12th day. Results Compared with induction group, the expression of osteoclasts, bone resorption lacuna, Calcr mRNA and Ctsk mRNA decreased in different dosage matrine group. The expression of TRAF6, c-fos and NFATc1 mRNA in different dosage groups of matrine was significantly lower than that in induction group (P < 0.01), and was dose-dependent. There was no significant difference in the expression of TRAF6, c-fos and NFATc1 mRNA between blank group and high dose group of matrine (P > 0.05). Compared with induction group, the expression of TRAF6, c-fos and NFATc1 protein in matrine low, middle and high dose groups decreased (P < 0.05), and it was dose-dependent. There was no significant difference in the expression of TRAF6, c-fos and NFATc1 between blank group and high dose group of matrine (P > 0.05). Conclusion Matrine may affect RANKL-induced osteoclasts differentiation by inhibiting the expression of TRAF6, c-fos and NFATc1.
李欣1 李芳2 王媛1 张宝玉1 张丽洁1. 苦参碱对RANKL诱导破骨细胞分化及TRAF6、c-fos、NFATc1表达水平的影响[J]. 中国医药导报, 2020, 17(1): 13-16,32.
LI Xin1 LI Fang2 WANG Yuan1 ZHANG Baoyu1 ZHANG Lijie1. Effect of matrine on RANKL-induced osteoclast differentiation and the expression of TRAF6, c-fos and NFATc1. 中国医药导报, 2020, 17(1): 13-16,32.
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