不同参数的重复经颅磁刺激对大鼠血清脑源性神经营养因子的影响

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摘要目的观察不同参数的重复经颅磁刺激（rTMS）对大鼠血清中脑源性神经营养因子（BDNF）的影响。方法 Sprague-Dawle大鼠共54只，分为Sham组（n = 18）、1 Hz rTMS组（n = 12）、10 Hz rTMS组（n = 12）和间断θ节律性刺激（iTBS）组（n = 12）。根据刺激强度，即静息运动阈值（rMT），分为Sham组、低强度组（80% rMT）和高强度组（120% rMT）进行实验，每组各6只。按照预先设定的参数进行rTMS持续刺激大鼠前额皮质5 d，并应用酶联免疫吸附试验（ELISA）检测大鼠血清中BDNF水平的变化。结果低强度1 Hz rTMS组BNDF表达显著高于Sham组，差异有统计学意义（P ＜ 0.05）。而高强度1 Hz rTMS组与Sham组比较，差异无统计学意义（P ＞ 0.05）。Sham组、低强度10 Hz rTMS和高强度10 Hz rTMS组BDNF水平比较，差异无统计学意义（P ＞ 0.05）。高强度iTBS组血清中BNDF表达明显低于Sham组，差异有统计学意义（P ＜ 0.05）。而低强度iTBS组与Sham组比较，差异无统计学意义（P ＞ 0.05）。结论强度是影响rTMS效果的重要因素，不同模式的rTMS对BDNF的效果随强度的不同而变化。

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	董国英陈霞周先举王林晓

关键词 ：重复经颅磁刺激, 间断&theta, 节律性刺激, 强度, 脑源性神经营养因子

Abstract：Objective To observe the effect of repetitive transcranial magnetic stimulation (rTMS) with different parameters on serum brain-derived neurotrophic factor (BDNF) in rats. Methods A total of 54 Sprague-Dawle rats were divided into the Sham group (n = 18), 1 Hz rTMS group (n = 12), 10 Hz rTMS group (n = 12), and the intermittent theta burst stimulation (iTBS) group (n = 12). According to the stimulation intensity (the resting motor threshold, rMT), each experiment contains of the Sham group, the low-intensity group (80% rMT) and the high-intensity group (120% rMT), six mice in each group. The prefrontal cortex of rats were treated with rTMS in consecutive five days. Enzyme linked immunosorbent assay (ELISA) was used to detect the change of the serum BDNF level of rats. Results The level of BNDF in the low-intensity 1 Hz rTMS group was significantly higher than that in the Sham group, and the difference was statistically significant (P ＜ 0.05), while there was no statistically significant between the high-intensity 1 Hz rTMS group and the Sham group (P ＞ 0.05). The levels of BDNF among the Sham group, the low-intensity 10 Hz rTMS group and the high-intensity 10 Hz rTMS group were no statistically significant (P ＞ 0.05). The level of BNDF in the high-intensity iTBS group was lower than that of the Sham group, and the difference was statistically significant (P < 0.05), while there was no statistically significant between the low-intensity iTBS group and the Sham group (P ＞ 0.05). Conclusion Intensity is a key parameter affecting the effect of rTMS, while the effect of rTMS in different modes on BDNF varies with the intensity.

Key words： Repetitive transcranial magnetic stimulation Intermittent theta burst stimulation Intensity Brain derived neurotrophic factor

基金资助:国家自然科学基金资助项目（81803498）；
江苏省常州市卫生计生委青年人才科技项目（QN201719）。

通讯作者: 王林晓（1986.6-），女，博士；研究方向：神经药理学。

作者简介: 董国英（1987.4-），女，硕士；研究方向：rTMS的基础研究与临床应用。

引用本文:

董国英陈霞周先举王林晓. 不同参数的重复经颅磁刺激对大鼠血清脑源性神经营养因子的影响[J]. 中国医药导报, 2019, 16(35): 8-10,35.
DONG Guoying CHEN Xia ZHOU Xianju WANG Linxiao. Effect of repetitive transcranial magnetic stimulation with different parameters on the expression of brain-derived neurotrophic factor in serum of rats. 中国医药导报, 2019, 16(35): 8-10,35.

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