NF-κB p65基因沉默对类风湿性关节炎滑膜细胞增殖凋亡的影响

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摘要目的探讨NF-κB p65基因沉默对类风湿性关节炎滑膜HFLS-RA细胞增殖及凋亡的影响。方法采用阳离子脂质体LipofectamineTM2000转染NF-κB p65的siRNA至HFLS-RA细胞中，利用RT-PCR和Western blot技术分别检测HFLS-RA细胞中NF-κB p65 mRNA和蛋白的表达情况。噻唑蓝MTT法和流式细胞仪检测细胞的增殖率和凋亡率。结果与空白对照组和阴性对照组比较，转染siRNA后的HFLS-RA细胞中NF-κB p65 mRNA、蛋白表达量和细胞增殖率均明显下降，而细胞凋亡率明显升高，差异均有统计学意义（均P ＜ 0.05）。结论 NF-κB p65基因在类风湿性关节炎滑膜HFLS-RA细胞的增殖和凋亡过程中起着重要作用，使其沉默后能够抑制滑膜HFLS-RA细胞增殖，诱导其凋亡。

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	罗海恩毛新展

关键词 ：类风湿性关节炎, 滑膜细胞, NF-&kappa, B, 细胞增殖, 细胞凋亡

Abstract：Objective To investigate the effect of NF-κB p65 gene silencing on proliferation and apoptosis of rheumatoid arthritis synovial HFLS-RA cells. Methods NF-κB p65 siRNA was transfected into HFLS-RA cells by cationic liposome LipofectamineTM2000, and the expression of NF-κB p65 mRNA and protein in HFLS-RA cells was detected by RT-PCR and Western blot respectively. The proliferation and apoptosis of HFLS-RA cells were separately detected by MTT and flow cytometry assay. Results Compared with the blank control group and the negative control group, the expression of NF-κB p65 mRNA, protein and cell proliferation rate in the HFLS-RA cells transfected with siRNA was decreased, while the apoptosis rate was increased, and the differences were statistically significant (all P ＜ 0.05). Conclusion NF-κB p65 gene plays an important role in the proliferation and apoptosis of synovial HFLS-RA cells in rheumatoid arthritis, and silencing its expression can inhibit the proliferation of synovial HFLS-RA cells and induce its apoptosis.

Key words： Rheumatoid arthritis Synovial cells NF-κB Cell proliferation Cell apoptosis

基金资助:国家自然科学基金资助项目（81371997）。

通讯作者: 毛新展（1972.3-），男，博士，主任医师；研究方向：骨关节。

作者简介: 罗海恩（1979.9-），男，中南大学湘雅二医院2011级骨科专业在读硕士研究生，副主任医师；研究方向：骨关节。

引用本文:

罗海恩毛新展. NF-κB p65基因沉默对类风湿性关节炎滑膜细胞增殖凋亡的影响[J]. 中国医药导报, 2017, 14(35): 8-11.
LUO Hai'en MAO Xinzhan. Effect of NF-κB p65 gene silencing on proliferation and apoptosis of rheumatoid arthritis synovial cells. 中国医药导报, 2017, 14(35): 8-11.

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